大黄素抑制软脂酸诱导的大鼠VSMCs炎性细胞因子的表达  

Inhibitory Effect of Emodin on Inflammatory Cytokines Expression Induced by Palmitic Acid in Rat Vascular Smooth Muscle Cells

作　　者：吴迪[1] 钟珊珊[1] 

机构地区：[1]遵义医学院药学院,贵州遵义563000

出　　处：《中国现代中药》2015年第2期109-113,共5页Modern Chinese Medicine

基　　金：遵义医学院硕士启动项目(F-669)

摘　　要：目的:探讨大黄素对软脂酸诱导的大鼠血管平滑肌细胞(VSMCs)炎症细胞因子CRP、TNF-α及i NOS的影响。方法:培养鉴定原代大鼠VSMCs,使用不同浓度的大黄素(1、10、50μmol·L^(-1))预孵育VSMCs 24 h,再以浓度为100μmol·L^(-1)的软脂酸刺激24 h,使用RT-PCR、western blot分别测定VSMCs CRP、TNF-α、i NOS的mRNA和蛋白的表达变化。结果:浓度为50μmol·L^(-1)的大黄素预孵育大鼠VSMCs 24 h后,与软脂酸组相比,CRP、TNF-α、i NOS的mRNA及蛋白的表达显著性降低,且差异有统计学意义(P<0.05)。结论:大黄素能浓度依赖性地抑制软脂酸诱导的大鼠VSMCs炎性因子的表达,可以通过抗炎而达到抗动脉粥样硬化的作用。Objective： To observe the effects of emodin on CRP, TNF-c~ and iNOS generations induced by palmitic acid in rat vascular smooth muscle cells （ VSMCs ）. Methods： After VSMCs were pretreated with different concentrations of emodin（ 1, 10, 50μmol·L^-1 ） for 24 h, VSMCs were treated with palmitic acid（100μmol·L^-1 ） for 24 h. At the indicated time, mRNA and protein expressions of CRP, TNF-α and iNOS in VSMCs were analyzed by RT-PCR, western blot, respectively. Results： In VSMCs, using 50 μmol·L^-1 of emodin for 24h before the stimulation with palmitic acid, mRNA and protein expressions of CRP, TNF-α and iNOS expressions were significantly decreased comparing with palmitic acid group. Conclusion： Emodin inhibits inflammatory cytokines expression induced by palmitie acid in VSMCs and has effect on atheroselerosis by its anti-inflammatory activity.

关 键 词：大黄素 软脂酸 血管平滑肌细胞 细胞因子 动脉粥样硬化 

分 类 号：R543.5[医药卫生—心血管疾病]