人脐带间充质干细胞移植在缺血缺氧性脑病治疗中的作用  

Effects of human umbilical cord mesenchymal stem cells transplantation on Wearing hypoxic-ischemic encephalopathy

作　　者：顾平[1] 张忠霞[2] 仇福成[1] 张君[3] 韩瑞[1] 张丽娜[1] 解冰川[1] 王文婷[1] 张振清[1] 王彦永[1] 王铭维[1] 闫宝勇[2] 

机构地区：[1]河北医科大学第一医院神经内科,石家庄050031 [2]河北省脑老化与认知神经科学实验室 [3]河北医科大学第一医院细胞治疗实验室,石家庄050031

出　　处：《中国综合临床》2015年第3期206-211,共6页Clinical Medicine of China

基　　金：河北省科技计划项目（12276102D）

摘　　要：目的观察人脐带间充质干细胞移植在缺血缺氧性脑病治疗中的作用。方法选取缺血缺氧性脑病患者共25例，随机分为干细胞移植组15例和对照组10例。对移植组患者进行静脉输注体外培养及无菌分离的人脐带间充质干细胞，对照组采用常规药物治疗。分别于移植前及移植后14、90、180d进行神经功能[美国国立卫生研究院卒中量表（NIHSS），Barthel指数（BI）]、锥体外系功能[帕金森病统一量表（UPDRS）]、认知功能及情绪反应[简易精神状态检查（MMSE）、汉密尔顿抑郁量表14项（HAMDl4）和HAMD24]的测评，评价人脐带间充质干细胞移植的临床疗效。结果治疗前两组各项功能差异无统计学意义，治疗后14、90、180d，移植组的各项评分比移植前有明显改善（NIHSS：F组内=4．372，P=0．031；F交互=4．175，P=0．038；F组间=3．897，P=0．045。BI：F组内=4．728，P=0．044；F交互=4．894，P=0．037；F组间=4．284，P=0．039。UPDRS：F组内=5．112，P=0．047；F交互=4．895，P=0．045；F组间=3．879，P=0．031。MMSE：F组内=5．135，P=0．039；F交互=3．213，P=0．036；F组间=4．184，P=0．045。HAMD14：F组内=3．977，P=0．049；F交互=4．587，P=0．038；F组间=4．381，P=0．041。HAMD24：F组内=3．845，P=0．033；F交互=4．125，P=0．035；F组间=3．547，P=0．034），且效果明显优于对照组，差异有统计学意义（P〈0．05）。结论人脐带源间充质干细胞移植治疗缺血缺氧性脑病安全有效，可以明显改善患者神经功能、锥体外系功能、认知功能及情绪。Objective To investigate the effects of human umbilical cord mesenchymal stem cells （hUCMSCs） transplantation on the treatment of hypoxic-iscbemic encephalopathy （HIE）. Methods A total of 25 HIE patients were randomly divided into stem cell transplantation group （15 case） and control group （10 cases）. The patients in transplantation group were given intravenous infusion of hUCMSCs, which isolated under sterile condition in vitro and cultured, while in control group were treated with routine drug treatment. Neurological function （ American National Institutes of Health Stroke Scale （ NIHSS ） , Barthel index （BI） ）, extrapyramidal function （ Unified Parkinson＇ s disease questionnaire （ UPDRS ） ）, cognition and emotional reaction（ The mini mental state examination（MMSE） , the 14 item Hamilton Depression Scale （HAMD14） and HAMD24） were all assessed before and after transplantation for 14 d ,90 d and 180 d respectively to evaluate the clinical efficacy of hUCMSCs transplantation. Results There was no significant difference between two groups in terms of each function before transplantation. The scores of transplantation group were all obviously improved after treatment for 14 d, 90 d and 180 d compared to that of before treatment, and the therapy effect in transplantation group was significantly better than that of the control group （ NIHSS ： Ftime = 4. 372, P = 0. 031 ; Ftime group = 4. 175, P = 0. 038 ; Fgroup = 3. 897, P = 0. 045 o BI ： Ftime = 4. 728, P = 0. 044 ; Ftime, group = 4. 894, P = 0. 037 ; Fgroup = 4. 284, P = 0. 039 o UPDRS ： Ftime = 5. 112, P = 0. 047 ; Ftime, group = 4. 895, P = 0. 045 ; Fgroup =3. 879,P =0. 031o MMSE： Ftime = 5. 135, P = 0. 039; Ftime.group = 3. 213, P = 0. 036; Fgroup = 4. 184, P = 0. 045.HAMD14 ： Ftime = 3. 977, P = 0. 049 ; Ftime.group = 4. 587, P = 0. 038 ; Fgroup = 4. 381, P = 0. 041. HAMD24 ： Ftime = 3. 845, P = 0. 033 ; Ftime.group = 4. 125, P = 0. 035 ; Fgroup = 3. 547, P = 0. 034 ）. Conclusion Transplantation

关 键 词：人脐带间充质干细胞 移植 缺血缺氧性脑病 

分 类 号：R722.12[医药卫生—儿科]