柚皮素防治去卵巢致大鼠骨质疏松症的实验研究  

Antiosteoporotic effects of naringenin on ovariectomy-induced osteoporosis in rat

作　　者：宋双红[1] 王德 莫易易 丁冲[1] 商澎[1] 

机构地区：[1]西北工业大学生命学院,西北工业大学特殊环境生物物理学研究所,空间生物实验模拟技术国防重点学科实验室,陕西西安710072 [2]西北濒危药材资源开发国家工程实验室,陕西师范大学生命科学学院,陕西西安710062

出　　处：《药学学报》2015年第2期154-161,共8页Acta Pharmaceutica Sinica

基　　金：陕西省自然科学基础研究项目(2014JQ3100)

摘　　要：研究柚皮素对去卵巢致大鼠骨质疏松骨代谢及相关组织生化指标的影响。选取32只3月龄未经产雌性Sprague-Dawley(SD)大鼠,随机分为4组:假手术组(Sham)、去卵巢模型组(OVX)、17β-雌二醇治疗组(E2)和柚皮素治疗组(Naringenin),每组灌胃相应药物,给药12周后收集大鼠24 h尿液,麻醉动物采集血清,处死后解剖大鼠取以下标本进行分析:称重考察各脏器系数;制作石蜡切片观察子宫病理变化;采用全自动生化分析仪对血清肝肾功能指标进行分析;ELISA等免疫学方法对骨转换指标血清骨钙素(BGP)和尿液脱氧吡啶啉(DPD)进行测定;采用三点弯曲实验对股骨生物力学性能进行分析;采用DEXA骨密度测定仪及Micro CT对骨矿密度(BMD)、骨矿含量(BMC)及干骺端骨小梁微组织结构进行测定。结果发现柚皮素可以对抗卵巢切除引起的大鼠体重增加,抑制骨转换指标BGP和DPD含量的升高,降低骨转换率,保持骨矿密度,改善骨应力和弹性模量等内在特性指标,同时骨小梁微结构也有了显著改善;另外,对肝肾功能未产生明显影响。可见柚皮素对去卵巢致大鼠骨质疏松有明显的防治作用,具有开发为妇女绝经后骨质疏松症治疗药物的潜在价值。To investigate the effect of naringenin on ovariectomy-induced postmenopausal osteoporosis comprehensively and systemically, thirty-two virgin Sprague-Dawley rats about 3-month-old were used and randomly divided into 4 groups： sham control group（Sham）, OVX control group（OVX）, naringenin treatment group and 17β-estradiol（E2） treatment group. After 12 weeks treatment with different drugs, 24 h urine were collected, organs were weighed and the organ indies were computed. Uterine pathological changes were observed by making paraffin section. Biochemical parameters and bone turnover markers： serum osteocalcin（BGP） and urine deoxypyridinoline（DPD） were analyzed with automatic biochemical analyzer or ELISA assay. Bone mineral density（BMD） and bone mineral content（BMC） were analyzed by DEXA, bone biomechanical properties was measured by three point bending test and the trabecular bone microarchitecture was evaluated by Micro CT. From the results, we can see that： the gaining of weight and the increasing of bone turnover markers such as serum BGP and urinary DPD could be inhibited by naringenin. The treatment could also enhance the bone strength and prevent the deterioration of trabecular microarchitecture, increase the bone volume, trabecular number and thickness, and decrease the trabecular space. The effects mentioned above were not accompanied with stimulating effects on uterus. Long-term using of naringenin had no obvious influence on other organs and the liver and kidney functions. The study suggests that naringenin had obvious antiosteoporotic effect on ovariectomized rats and it had the potential value for the treatment of postmenopausal osteoporosis.

关 键 词：骨质疏松 柚皮素 卵巢切除术 骨钙素 脱氧吡啶啉 

分 类 号：R963[医药卫生—微生物与生化药学]