G蛋白偶联受体激酶4对氧化低密度脂蛋白诱导的血管平滑肌细胞增殖的影响  

Effects of G protein-coupled receptor kinase 4 on proliferation in vascular smooth muscle cells induced by oxidized low density lipoprotein

作　　者：徐雪飞[1] 李美香[1] 任红梅[1] 韩愈[1] 陈彩宇[1] 周林[1] 曾春雨[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所心血管内科,重庆市心血管病研究所,重庆400042

出　　处：《第三军医大学学报》2015年第3期248-251,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金(81070559)~~

摘　　要：目的研究G蛋白偶联受体激酶4(GRK4)对氧化低密度脂蛋白(ox-LDL)诱导的血管平滑肌细胞增殖的影响。方法以大鼠胸主动脉平滑肌细胞株(A10细胞)为靶细胞,检测在不同浓度的ox-LDL(10、20、30、40、60、80 mg/L)刺激下A10细胞的增殖情况;观察ox-LDL(40 mg/L)刺激下A10细胞GRK4表达变化;用siRNA干扰A10细胞GRK4表达后,检测ox-LDL对A10细胞增殖的影响。Western blot检测GRK4蛋白表达,CCK-8检测细胞增殖活力。结果用不同浓度ox-LDL刺激A10细胞,发现ox-LDL(40 mg/L)刺激下细胞增殖幅度可达78.3%(P<0.05),且细胞GRK4蛋白表达增加[实验组(0.367 9±0.049 1),对照组(0.193 0±0.038 5),P<0.05];与对照组相比,用siRNA干扰A10细胞GRK4表达后ox-LDL对A10细胞增殖的影响明显减弱[对照组(1.050 1±0.302 9),ox-LDL刺激组(1.929 1±0.390 0),siRNA组(1.403 2±0.164 4),P<0.05]。结论 ox-LDL可增强GRK4表达,干扰细胞GRK4表达后可减弱ox-LDL诱导的大鼠平滑肌细胞的增殖。Objective To determine the effect of G protein-coupled receptor kinase 4（ GRK4） on the proliferation of vascular smooth muscle cells（ VSMCs） induced by oxidized low density lipoprotein（ ox-LDL）. Methods After the rat thoracic aortic smooth muscle cell line A10 was treated by ox-LDL at10,20,30,40,60 and 80 mg / L respectively,the cell viability was detected by CKK-8 assay. Western blotting was used to observe the GRK4 expression in the A10 cells treated by 40 mg / L ox-LDL and in the A10 cells transfected with GRK4 siRNA followed by 40 mg / L ox-LDL treatment. The proliferation of A10 cells transfected with GRK4 siRNA and 40 mg / L ox-LDL treatment were also observed by CCK-8 assay. Results Ox-LDL of 40 mg / L increased the proliferation of A10 cells with a maximal proliferative amplitude of 78. 3%（ P〈0. 05） and enhanced the protein expression of GRK4 when compared with control group（ 0. 367 9 ±0. 049 1 vs 0. 193 0 ± 0. 038 5,P〈0. 05）. Compared with the control group（ 1. 050 1 ± 0. 302 9）,the proliferation of A10 cells treated with ox-LDL（ 1. 929 1 ± 0. 390 0） was significantly decreased in GRK4 siRNA transfected cells（ 1. 403 2 ± 0. 164 4, P〈0. 05）. Conclusion Ox-LDL enhances GRK4 expression,and further improves the proliferation of smooth muscle cells.

关 键 词：GRK4 OX-LDL 血管平滑肌细胞 增殖 

分 类 号：R322.12[医药卫生—人体解剖和组织胚胎学] R329.28[医药卫生—基础医学]