大鼠脊髓损伤后神经元再生研究  

Neuron regeneration after spinal cord injury in rats

作　　者：南国新[1] 匡红 汪九龄[1] 宿玉玺[1] 毕杨[1] 何波[1] 蔡文全[1] 陈凯[1] 李程[1] 

机构地区：[1]重庆医科大学附属儿童医院骨二科,儿童发育疾病研究教育部重点实验室干细胞生物学与治疗研究室 重庆市儿科学重点实验室 重庆市儿童发育重大疾病诊治与预防国际科技合作基地,400014 [2]解放军第四五二医院检验科

出　　处：《中华创伤杂志》2015年第2期164-168,共5页Chinese Journal of Trauma

基　　金：国家自然科学基金资助项目（81272172）;国家临床重点专科建设资助项目（2013544）

摘　　要：目的 观察成年大鼠脊髓损伤后是否存在神经元再生. 方法 36只Wistar成年雌性大鼠按随机数字表法分为正常对照组（6只）和脊髓损伤组（30只）.采用Allen打击模型（25 g·cm）在T10段造成急性脊髓损伤,于损伤后3d、1,2,3,4周进行取材.取材前72 h开始经腹腔注射BrdU （300 mg·kg^-1·d^-1）,每12 h注射1次.取距离损伤中心10 mm内脊髓行NF-200和BrdU免疫荧光双标,观察其表达阳性细胞数量. 结果 正常对照组可见少量BrdU阳性细胞,未发现BrdU和NF-200双标阳性细胞.脊髓损伤组伤后3d可见大量BrdU阳性细胞出现;1周时BrdU阳性细胞数量达到高峰,并可见少量BrdU和NF-200双标阳性细胞;2周时BrdU和NF-200双标阳性细胞最多见,主要集中在距离损伤区约3～5 mm处;3周时BrdU和NF-200双标阳性细胞罕见;4周时均未发现BrdU和NF-200双标阳性细胞. 结论 大鼠脊髓损伤后在损伤部位附近存在神经元再生,为干细胞修复脊髓损伤提供新的思路.Objective To observe the neuronal regeneration after spinal cord injury in adult rats.Methods Thirty-six adult Wistar rats were randomly divided into normal control group （n =6） and spinal cord injury group （n =30）.Allen＇s weight-drop method （25 g/cm） was adopted to produce acute spinal cord injury at T10 segment.Rats were sacrificed at 3 days and 1,2,3,and 4 weeks postinjury.BrdU was administered intraperitoneally to the rats 72 hours before operation,once per 12 hours.Double immunofluorescence staining of NF-200 and BrdU was performed at spinal sites within 10 mm away from the injury to detect the positive cells in both groups.Results A few BrdU-positive cells were founded in the normal control group,with no BrdU/NF-200 positive cells.In spinal cord injury group,BrdU-positive cells were abundant at day 3 and peaked at week 1 along with few Brdu/NF-200 positive cells ; Brdu/NF-200 positive cells showed a peak increase at week 2 with majority accumulated at area 3-5 mm away from the injury,were rare at week 3 and none at week 4.Conclusion Neuronal regeneration is detected in the vicinity of the injury,which provides new ideas for induction of endogenous stem cells to treat spinal cord injury.

关 键 词：脊髓损伤 神经元 干细胞 大鼠 

分 类 号：R651.2[医药卫生—外科学]