Graves'病患者CD4^+CD25^+调节性T细胞功能初探  

Study on the function of CD4^+ CD25^+ regulatory T cells in patients with Graves' disease

作　　者：孙伟莉[1,2] 申勇[2] 李卫鹏[2] 袁媛[2] 张林杰[1] 

机构地区：[1]安徽医科大学基础医学院免疫学教研室,合肥230032 [2]安徽蚌埠医学院第一附属医院核医学科,蚌埠233004

出　　处：《安徽医科大学学报》2015年第2期202-205,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省高校省级自然科学基金(编号:KJ2011A167)

摘　　要：目的探讨调节性T细胞(Treg)在Graves’病(GD)发生中所起的作用。方法流式细胞术检测20例GD患者(GD组)和10例健康对照者(健康对照组)外周血Treg数量。3H掺入试验测定Treg与效应T细胞(Teff)共培养环境中的Treg对其增殖抑制作用,ELISA法测定白细胞介素-10(IL-10)、白细胞介素-2(IL-2)等细胞因子的浓度。结果Treg所占CD4+细胞的比例在GD组与健康对照组中差异无统计学意义。GD组的Treg抑制功能与健康对照组相比显著降低。两组之间的细胞因子浓度有一定的差异,GD组IL-10水平显著低于健康对照组,IL-2和白细胞介素-17(IL-17)水平显著高于健康对照组。结论 GD患者的Treg有功能缺陷,与甲状腺自身免疫性疾病的发病有一定的关系。Treg在GD中的诊断价值以及病理生理机制值得进一步探讨。Objective To investigate the role of regulatory T cells（ Tregs） in Graves’ disease （ GD） . Methods Treg number was assessed by flow cytometric analysis in samples from 20 GD patients and 10 healthy controls. The inhibitory effect of Treg on T effector cells（Teff） was assayed by 3H thymidine proliferation experiment. IL-10,IL-2 and other cytokine levels were determined by ELISA in conditioned media from the co-cultures. Results No differ-ences were found in the frequency of Tregs as a percentage of CD4 ＋ cells between GD and healthy controls. Com-pared with the healthy controls, inhibitory function of Treg from patients with GD decreased significantly. Cytokine secretion between two groups also had significant difference. IL-10 secretion of healthy control group was signifi-cantly higher than patients group, while secretion of IL-2 and IL-17 was significantly lower than patients group. Conclusion Tregs from GD patients are partly dysfunctional, possibly explaining their autoimmunity. Future work will elucidate the diagnostic potential and pathophysiology of Tregs in GD.

关 键 词：Graves’病 调节性T细胞 效应T细胞 

分 类 号：R392.6[医药卫生—免疫学]