Olig2对胚胎干细胞向神经前体细胞分化过程中Nestin蛋白表达的影响  

The effect of oligodendrocyte transcription factor 2 on the expression of nestin during the differentiation of mouse embryonic stem cells into neural precursor cells

作　　者：罗梦娇[1] 王贝[1,2] 郭瑞[1] 曲学彬[1] 王会平[1] 张强[1] 董富兴[1] 任传路 姚瑞芹[1] 

机构地区：[1]徐州医学院生物学教研室,徐州221009 [2]苏州解放军第100医院中心实验室,苏州215007

出　　处：《神经解剖学杂志》2015年第1期91-94,共4页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(81271345;81302519);江苏省自然科学基金(BK20131132;BK20130221);徐州市社会发展科技项目(XZZD1324);徐州医学院院长基金(2012KJ07)

摘　　要：目的:探讨碱性螺旋-环-螺旋家族的少突胶质细胞转录因子2(Olig2)对小鼠胚胎干细胞(mESCs)向神经前体细胞(NPCs)分化过程中巢蛋白(Nestin)表达的影响。方法:将mESCs分为正常组、空载体组和Olig2转染组,用含有RA、Purmorphamine、bF GF和PDGF-AA的培养基将其诱导分化为NPCs;分别在诱导分化的第11 d和14 d,用免疫细胞化学荧光染色和蛋白免疫印迹法检测其神经前体细胞特异性标记物巢蛋白(Nestin)的表达情况。结果:经Olig2-GV218慢病毒转染后的mE SCs,Olig2蛋白的表达较正常组和空载体组明显增强;诱导分化后11 d,三组细胞Nestin蛋白表达无明显区别;14 d时,正常组和空载体组仍能检测到较高的Nestin蛋白表达,而Olig2转染组Nestin蛋白的表达明显减弱。结论:mESCs向NPCs分化过程,过表达Olig2可降低Nestin蛋白的表达。Objective： To investigate the effect of a basic helix-loop-helix （bHLH） oligodendrocyte transcription factor 2 on the expression of nestin during the differentiation of mouse embryonic stem cells into neural precursor cells. Methods ： The mESCs were divided into three groups ： mESCs normal group, mESCs empty vector group and mESCs Olig2 transfect- ed group ; They were cultured in the presence of RA, Purmorphamine, bFGF and PDGF-AA, then induced to differentia- ted into NPCs; at day 11 and 14 of induction, the expression of nestin was detected as a marker of NPCs, using immunofluorescence staining and Western Blot. Results： The mESCs were infected with Olig2-GV218, and the expression of Olig2 in the Olig2 transfected group was higher than that in the normal group and the empty vector group. At day 11, the expression of nestin among the three groups had no significant difference. At day 14, high levels of nestin were still detectable in the normal group and the empty vector group, but not in the Olig2 transfected group. Conclusion： Olig2 overexpression may reduce the expression of nestin during the differentiation of mESCs into NPCs.

关 键 词：小鼠胚胎干细胞 神经前体细胞 过表达 少突胶质细胞转录因子2 巢蛋白 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]