维甲酸诱导小鼠iPS细胞向神经细胞分化  

Mouse induced pluripotent stem cells differentiate into neurons by retinoic acid

作　　者：范文娟[1] 马永超[1] 陈旭东[1] 袁磊[1] 饶淑梅[1] 王福青[1] 

机构地区：[1]漯河医学高等专科学校分子医学实验室,漯河462002

出　　处：《神经解剖学杂志》2015年第1期103-108,共6页Chinese Journal of Neuroanatomy

基　　金：河南省自然科学基金(12B320017;12B180016);河南省科技厅年度研究计划项目(122102310495);漯河医学高等专科学校基金(2013-S-LMC06)

摘　　要：目的:研究小鼠诱导性多能干细胞(induced pluripotent stem cells,iP S细胞)在体外维甲酸(retinoic acid,RA)的诱导下能否分化成功能性的神经细胞。方法:首先将iPS细胞悬浮培养形成拟胚体,利用RA将其诱导分化成神经前体细胞,然后利用免疫荧光染色技术观察小鼠iPS细胞在体外分化成神经细胞以及突触发生的形态特征。结果:小鼠iPS细胞在RA的诱导下不但能够分化为成熟神经元与胶质细胞还可以观察到树突棘及突触连接的形成。结论:RA作为一种作用很强的分化诱导剂,可以有效的诱导小鼠iPS细胞分化为功能性的神经元和神经胶质细胞,这对其进行后续的神经发育的机制、药物筛选以及自体iPS细胞移植的研究具有重要意义。Objective： To study mouse induced pluripotent stem （iPS） cells whether can be differentiated into functional neurons induced by retinoic acid （RA）. Methods： First we pre-differentiated mouse iPS cells into neural precursor cells by using RA-induction after embryoid body （EB） formation, then observed the morphological characteristics of neurons formation and synaptogenesis synapse using immunofluorescence staining in vitro. Results： We found mouse iPS ceils not only could differentiate to mature neurons and glial cells, also could form dendritic spines and synaptic connections by morphological analyses. Conclusion： Our results revealed that the processes involved in the formation of synapses in mouse iPS cells differentiated into functional neurons and glia, which may have important implications for neurodevelop- mental studies, safety pharmacological studies and autologous cell transplantation.

关 键 词：IPS细胞 干细胞分化 突触形成 树突棘 小鼠 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]