慢性荨麻疹患者外周血白细胞介素9和转录因子PU.1的表达及意义  

Expressions of interleukin-9 and the transcription factor PU.1 in peripheral blood of patients with chronic urticaria and their significance

作　　者：张明海 戴前梅 胡春艳 陈晨 陈朋 

机构地区：[1]安徽省第二人民医院皮肤科,合肥230000

出　　处：《中华皮肤科杂志》2015年第2期108-111,共4页Chinese Journal of Dermatology

摘　　要：目的 探讨白细胞介素9（IL-9）和Th9细胞与慢性荨麻疹的关系.方法 收集门诊慢性荨麻疹患者30例,另30例健康志愿者作为对照组.用酶联免疫吸附试验（ELISA）检测外周血IgE水平,流式细胞仪检测外周血CD4＋ IL-9＋细胞比例,实时荧光定量PCR检测外周血单一核细胞（PBMC）IL-9、PU.1 mRNA的表达.两组间定量资料比较采用独立样本t检验,相关性检验采用Pearson分析.结果 慢性荨麻疹患者PBMCIL-9及PU.1 mRNA的表达水平均显著高于健康对照组（4.44±1.90比3.20±1.78,3.26±1.59比2.34±1.47,t=2.60,2.34,均P＜0.05）.慢性荨麻疹患者外周血CD4＋IL-9＋细胞比例[（0.63±0.44）％]、IgE水平[（82.04±31.72） IU/ml]与健康对照组[（0.22±0.12）％、（60.74±28.26） IU/ml]比较均明显升高（t=5.04,2.75,均P＜0.01）.慢性荨麻疹患者外周血CD4＋IL-9＋细胞比例、IL-9及PU.1 mRNA与IgE水平均呈正相关（R=0.596,0.767,0.746,均P＜0.01）.结论 Th9细胞和IL-9可能参与慢性荨麻疹的发病.Objective To investigate the relationship of interleukin-9 （IL-9） and the transcription factor PU.1 with chronic urticaria.Methods Thirty patients with chronic urticaria （patient group） and 30 healthy volunteers （control group） were included in this study.Venous blood samples were collected from these subjects.Enzyme-linked immunosorbent assay （ELISA） was performed to detect serum levels of IgE,flow cytometry （FCM） to determine the percentage of CD4＋IL-9＋ T cells,real-time fluorescence-based quantitative PCR （RTFQ-PCR） to measure the mRNA expressions of IL-9 and PU.1 in peripheral blood mononuclear cells （PBMCs）.Measurement data were compared between the two groups by independent-samples t test,and relationship among these parameters was assessed by Pearson correlation analysis.Results The expressions of IL-9 and PU.1 mRNAs in patients with chronic urticaria were significantly higher than those in healthy controls （4.44 ± 1.90 vs.3.20 ± 1.78,t =2.60,P〈 0.05; 3.26 ± 1.59 vs.2.34 ± 1.47,t =2.34,P 〈 0.05）.Compared with the control group,the patient group showed increased percentage of CD4＋IL-9＋ T cells in peripheral blood （0.63% ± 0.44% vs.0.22% ± 0.12%,t =5.04,P 〈 0.01） and serum IgE levels （（82.04 ± 31.72） vs.（60.74 ± 28.26） IU/ml,t =2.75,P 〈 0.01）.The percentage of CD4＋IL-9＋ T cells and levels of IL-9 and PU.1 mRNAs in peripheral blood were all positively correlated with serum levels of IgE in these patients （r =0.596,0.767,0.746,respectively,all P 〈 0.01）.Conclusion T helper type 9 （Th9） cells and IL-9 may take part in the occurrence of chronic urticaria.

关 键 词：荨麻疹 白细胞介素9 TH9细胞 

分 类 号：R758.24[医药卫生—皮肤病学与性病学]