早期斑秃皮损处炎症细胞因子及凋亡因子的异常表达  

Abnormal expressions of inflammatory cytokines and apoptosis-related factors in lesions of early alopecia areata

作　　者：蔡泽明[1] 赵莹[2] 张斌[3] 巩毓刚[3] 西兰[3] 杨建[3] 章星琪[3] 

机构地区：[1] 南昌大学第三附属医院皮肤科,332000 [2] 山东菏泽市立医院皮肤科 [3] 中山大学附属第一医院皮肤科

出　　处：《中华皮肤科杂志》2015年第2期128-131,共4页Chinese Journal of Dermatology

摘　　要：目的 探讨早期斑秃皮损浅层、深层及斑秃生长期毛囊凋亡因子及炎症细胞因子的表达.方法 收集25例早期斑秃皮损及15例正常头皮病理取材组织标本,通过荧光定量PCR检测标本中凋亡基因p53、caspase3、Fas和生存素、bcl-2以及炎症细胞因子白细胞介素（IL）-4、IL-10、干扰素γ（IFN-γ）、IL-12 mRNA表达水平,免疫组化检测皮损中生长期毛囊P53的蛋白质表达.结果 斑秃生长期毛囊促凋亡因子caspase3、P53、Fas mRNA表达水平较对照组升高,变化倍数分别为6.78、8.01、9.74,差异有统计学意义（均P＜0.05）.抑制凋亡因子bcl-2、生存素mRNA较正常降低,变化倍数分别为0.08、0.03（均P＜0.01）,炎症细胞因子无改变.斑秃浅层Th1因子IFN-γ、IL-12 mRNA较正常水平升高,变化倍数分别为2.75、85.67（P值分别＜0.05、0.01）.Th2因子IL-10表达水平降低,变化倍数为0.002（P＜ 0.01）.而且,斑秃皮损浅层IL-10及IL-12 mRNA改变幅度高于深层（P值分别＜ 0.01、0.05）.免疫组化显示斑秃皮损生长期毛囊每100个细胞中p53阳性细胞数较对照组升高（t=23.79,P＜ 0.01）.结论 斑秃生长期毛囊凋亡因子升高,抑制凋亡因子表达下降提示凋亡因子在斑秃发病中起一定作用.Objective To detect the expressions of apoptosis-related factors and inflammatory cytokines in superficial and deep layers of as well as anagen hair follicles in lesions of early alopecia areata （AA）.Methods Scalp biopsy samples were collected from 25 patients with early AA and 15 healthy human controls.Fluorescence-based quantitative PCR was performed to detect mRNA expressions of apoptosis-related genes p53,caspase 3,Fas,survivin and bcl-2,as well as those of inflammatory cytokines interleukin （IL）-4,IL-10,IL-12 and interferon （IFN）-γ.An immunohistochemical assay was conducted to measure the expression of p53 protein in anagen hair follicles.Results Compared with control skin samples,anagen hair follicles in AA lesions showed significantly increased mRNA expression levels （expressed as 2-△△Ct） of pro-apoptotic factors caspase 3,p53 and Fas （6.78,8.01,9.74,respectively,all P 〈 0.05）,but decreased mRNA expression levels of antiapoptotic factors bcl-2 and survivin （0.08 and 0.03 respectively,both P 〈 0.01）,and similar mRNA expression levels of inflammatory cytokines.There was a significant increase in mRNA expression levels of Th1 cytokines IFN-γ and IL-12 （2.75 vs.1.00,P 〈 0.05; 85.67 vs.1.00,P 〈 0.01）,but a significant decrease in the expression level of the Th2 cytokine IL-10 （0.002 vs.1.000,P 〈 0.01） in superficial layers of AA lesions compared with those of normal control skin.The degree of changes in mRNA expression levels of IL-10 and IL-12 was significantly higher in superficial layers than in deep layers of AA lesions （P〈0.01 and 0.05 respectively）.The immunohistochemical assay showed that the number of p53-positive cells per 100 cells in anagen hair follicles of AA lesions was higher than that in those of control skin （t =23.79,P 〈 0.01）.Conclusions Anagen hair follicles in AA lesions exhibit high expressions of pro-apoptosis factors,but low expressions of antiapoptotic factors,suggesting that apoptotic factors play a role in the occurrence of A

关 键 词：斑秃 细胞因子类 凋亡诱导因子 肿瘤抑制蛋白质P53 

分 类 号：R758.71[医药卫生—皮肤病学与性病学]