COMP-Ang-1促去细胞神经血管化的放射显影法评价  

Radiography evaluation of enhanced vascularization of COMP-Ang-1 on acellular nerve

作　　者：丘龙海 何波[2] 朱昭炜[2] 胡军[1] 刘小林[2] 朱家恺[2] 

机构地区：[1]中山大学附属第一医院东院上肢骨科,广州510800 [2]中山大学附属第一医院显微创伤骨科

出　　处：《中华显微外科杂志》2015年第1期56-60,共5页Chinese Journal of Microsurgery

基　　金：广东省自然科学基金博士启动项目（1045100890100590,S201204006336）;高等学校博士学科点专项科研基金（20120171120075）

摘　　要：目的 用放射显影法观察去细胞神经血管化方式及评价COMP-Ang-l促去细胞神经血管化效果. 方法 2013年3月-2014年6月,用化学试剂萃取法获得去细胞神经,36只雌性大鼠随机分成2组,每组18只,分别用去细胞神经（对照组）或去细胞神经加COMP-Ang-1（实验组）修复1 cm长的坐骨神经缺损大鼠模型：于术后7、14、21 d用氧化铅经颈动脉灌注取材,用放射显影法扫描去细胞神经,获得平面二维图,观察去细胞神经血管化方式及COMP-Ang-1的促进血管再生效果. 结果 术后7、14和21 d,实验组血管密度分别为2701.60±318.93、3309.21±381.31和4787.33±251.09,分别高于其对照组（925.40±106.22,P=0.03;2832.70±189.23,P=0.021;3469.36±232.10,P=0.030）;术后7、14d,实验组血管面积（μm^2）（分别为9231.03±581.91、15561.13±697.73）高于对照组（分别为4839.01±101.01和6811.07±250.05）（分别为P=0.043,P=0.049）,差异有统计学意义（P＜0.05）. 结论 去细胞神经血管化起始于吻合口两端,逐步向桥接体中间部分生长;COMP-Ang-1在一定程度上能促进去细胞神经血管化.Objective To observe the way of vascularization of acellular nerves and evaluate the enhanced vascularization of using COMP-Ang-1 into acellular nerve on bridging sciatic nerve gaps by radiography.Methods From March,2013 to June,2014,acellular nerves were harvested by chemical extraction.Thirty-six female rats weighing 200-250 g were randomly divided into 2 groups：18 animals with 1 cm long sciatic nerve lesions were repaired by nerve grafting （control group）,18 animals with 1 cm long sciatic nerve lesions were repaired by nerve grafting and COMP-Ang-1 were administrated after surgery.Grafts were harvested after perfusion of lead oxide （carotid artery） on day 7,day 14 and day 21 postoperatively.Radiography was performed to capture the two dimensional image.The rules of vascularization of acellular nerve and the enhanced effects of COMP-Ang-1 on vascularization were evaluated.Results The density of vessels in COMP-Ang-1 group were higher than control group after 7 days （2701.60 ± 318.93 vs.925.40 ± 106.22,P =0.030）,14 days （3309.21 ± 381.31 vs.2832.70 ± 189.23,P =0.210） and 21 days （4787.33 ± 251.09 vs.3469.36 ± 232.10,P =0.030） postoperatively; the area of vessels in COMP-Ang-1 group were higher than control group after 7 days （9231.03 ± 581.91 μm2 vs.4839.01 ± 101.01 μm^2,P =0.043） and 14 days （15561.13 ± 697.73 vs.6811.07 ± 250.05,P =0.049） postoperatively.Conclusion COMP-Ang-1 can enhance the vascularization of acellular nerves fairly.

关 键 词：去细胞神经 寡聚硫酸软骨-促血管生成素-1 血管化 放射显影术 神经修复 

分 类 号：R651.3[医药卫生—外科学]