KIR3DL1启动子区域CpG岛甲基化对抗原表达的影响  

Effects of methylated CpG islands in promoter region on the expression of KIR3DL1 protein

作　　者：陶苏丹[1] 和艳敏[1] 董丽娜[1] 章伟[1] 何吉[1] 朱发明[1] 吕杭军[1] 

机构地区：[1]浙江省血液中心,卫生部血液安全研究重点实验室,杭州310006

出　　处：《中华微生物学和免疫学杂志》2015年第1期14-17,共4页Chinese Journal of Microbiology and Immunology

基　　金：浙江省自然科学基金（LY12H10001和LY14H100001）;浙江省医药卫生科学研究基金（2012KYA061）;国家自然科学基金（81401732）

摘　　要：目的：探索KIR3DL1启动子区域CpG岛甲基化对抗原表达的影响。方法选择抗原高表达KIR3DL1＊01502和低表达KIR3DL1＊005样本，分别测定启动子区域碱基序列和启动子区CpG岛甲基化程度。对携带有KIR3DL1＊01502、KIR3DL1＊005的NK细胞分别采用5-aza进行去甲基化处理，利用流式细胞仪检测KIR3DL1抗原。结果 KIR3DL1＊01502和KIR3DL1＊005启动子区域－65和－269位存在碱基差异，这导致它们的启动子区域有两个不同的CpG岛。 KIR3DL1＊01502启动子区CpG岛高度甲基化，去甲基化后相应的细胞表面抗原表达显著增加。而携带KIR3DL1＊005的细胞去甲基化处理后，抗原表达没有明显变化。结论 KIR3DL1＊01502启动子区域CpG岛甲基化影响抗原的表达，但是不同抗原表达模式的KIR3DL1等位基因可能存在不同的调控机制。Objective To investigate the effects of methylated CpG islands in the promoter region on the expression of killer cell immunoglobulin-like receptor 3DL1 （KIR3DL1）.Methods Three voluntary unpaid blood donors carrying high expression allele KIR 3DL1＊01502 and three donors carrying low expres-sion allele KIR3DL1＊005 were recruited in this study .The nucleotide sequences and the methylated CpG islands in the promoter regions of KIR 3DL1＊01502 allele and KIR3DL1＊005 allele were analyzed .The NK cells expressing KIR3DL1＊01502 and KIR3DL1＊005 were respectively treated with 5-aza for the dem-ethylation of CpG islands within the promoters .The expression of KIR3DL1 protein on the surface of NK cells was measured with flow cytometer .Results Two differences at nucleotide sites -65 and -269 were detected within the promoter regions of KIR3DL1＊01502 and KIR3DL1＊005, resulting in two distinct CpG islands.The CpG islands within the promoter of KIR 3DL1＊01502 allele were highly methylated .The ex-pression of KIR3DL1 protein on NK cells which carried KIR 3DL1＊01502 allele was significantly increased after the demethylation of CpG islands .However , the treatment of demethylation had no significant effects on the expression KIR3DL1 protein on NK cells harboring KIR3DL1＊005 allele.Conclusion The methylated CpG islands within the promoter of KIR 3DL1＊01502 allele affected the antigen expression on the surface of NK cells.Different KIR3DL1 alleles might show different mechanisms in regulating antigen expression .

关 键 词：杀伤细胞免疫球蛋白样受体 甲基化 启动子 

分 类 号：R392[医药卫生—免疫学]