茜草对大鼠骨骼生物力学性能的影响  

Effects of Madder on Bone Biomechanical Property in Rats

作　　者：吴晨晨[1] 杨小雯[1] 王文龙[1] 王姗姗[1] 曹丹丹[1] 马烽[1] 王建国[1] 路浩[1] 赵宝玉[1] 

机构地区：[1]西北农林科技大学动物医学院,杨凌712100

出　　处：《生物医学工程学杂志》2015年第1期110-115,共6页Journal of Biomedical Engineering

基　　金：西北农林科技大学博士科研启动金资助项目(Z111021305);国家自然科学基金青年基金资助项目(K305021306);博士后面上资助项目(K308021401)

摘　　要：茜草对骨骼具有红染效果,但不知道采食茜草是否会改变骨生物力学性能和骨中无机质的含量。因此,本文建立茜草饲喂大鼠的动物模型,采用万能材料力学检测仪、电感耦合等离子质谱和光谱仪检测大鼠股骨的生物力学性能和骨中无机质的含量,并应用高效液相色谱分析骨中红色物质。其结果发现:饲喂茜草6个月后,10%和15%组大鼠股骨骨干生物力学性能参数值显著高于对照组;骨中钙、镁和锌的含量显著高于对照组大鼠;但15%组大鼠肾脏淤血和充血,血液中尿素氮和肌酐含量与对照组大鼠之间差异显著;经分析得出大鼠骨骼中的红色物质是一种类茜草素的物质。以上结果说明:饲喂茜草6个月后,日粮中10%含量的茜草对大鼠无毒性,并能加强大鼠骨骼生物力学性能和增加骨中无机质的含量。Bones are stained into red color with feeding madder,but we do not know whether the fed madder can change the bone biomechanical properties and bone mineral contents in animals.In this research,we established a rat model with feeding madder.The bone biomechanical properties were detected by universal material mechanics,bone mineral contents were detected by inductively coupled plasma mass spectrometry and spectrometer,and red color material in bone was analyzed by high performance liquid chromatography.The results showed that bone biomechanical parameters in femur diaphysis in the 10% and 15% group rats were significantly higher than those in the control group after feeding madder for 6months.The level of calcium,magnesium and zinc in femur diaphysis in 10% and15% group rats were higher than those in the control group after feeding madder for 6 months.However,it was shown that the kidney congestion andhyperemia and the level of blood urea nitrogen and creatinine in the 15% group rats were significantly different compared to those in the control group rats after feeding madder for 6months.The red colored material in bone is related to alizarin analyzed with high-performance liquid chromatography.The conclusion could be drawn that feeding 10% madder in diet was not toxic to the rats fed for 6months,and it could improve bone biomechanical properties and increase bone mineral elements.

关 键 词：茜草 大鼠 骨生物力学 股骨 

分 类 号：R285.5[医药卫生—中药学]