γ-射线对细粒棘球蚴原头节在小鼠体内生长的抑制作用  

Inhibition Role of γ-Ray on Echinococcus Granulosus Protoscoleces in Mice In Vivo

作　　者：袁庆[1] 李波[2] 姜世平[1] 罗兰云[3] 李耿[1] 赵强[1] 张利勇[1] 

机构地区：[1]雅安市人民医院普外科,四川雅安625000 [2]泸州医学院附属医院肝胆外科,四川泸州646000 [3]四川省人民医院肝胆外科,四川成都610072

出　　处：《中国普外基础与临床杂志》2015年第2期139-143,共5页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的探讨γ-射线对细粒棘球蚴原头节生长的抑制作用。方法在体外培养的基础上,利用不同剂量的γ-射线对细粒棘球蚴原头节进行照射,并将照射后的原头节种植于小白鼠腹腔,4个月后进行剖腹观察,对不同照射剂量组(分别为10 Gy、20 Gy、40 Gy、80 Gy)原头节种植后所形成棘球蚴的发生情况、重量、组织结构进行分析,并与不照射(对照组)作对比。结果经过照射后,原头节种植于小白鼠腹腔后其棘球蚴发生率逐步降低,对照组为100%,10 Gy组为80.0%,20 Gy组为33.3%,40 Gy组为33.3%,80 Gy组为26.7%。剖腹称重后,各组棘球蚴重量中位数比较,10 Gy组(35.80 mg)、20 Gy组(0.00 mg)、40 Gy组(0.00 mg)及80 Gy组(0.00 mg)与对照组(157.80mg)相比,均明显下降,差异有统计学意义(P<0.001)。实验组所形成的棘球蚴发生钙化。结论γ-射线的照射可以抑制原头节形成棘球蚴,并可以导致所形成的棘球蚴结构破坏,加快棘球蚴坏死、钙化的转归过程。Objective To investigate the effect of γ-ray irradiation on echinococcus protoscoleces before abdominal cavity implantation in mice. Methods Echinococcus protoscoleces were cultured in vitro, and then divided into 5 groups, four groups received γ-ray irradiation with 10 Gy, 20 Gy, 40 Gy, and 80 Gy, respectively. The remaining control group was not irradiated. The protoscoleces were then implanted into the abdominal cavity of mice. Four months later, the incidence of echinococcosis was measured by detection of echinococcus antibody in the blood and then abdominal laparotomy was performed to isolate intraabdominal cysts, and the weight and structure of the echinococcus generated from the protoscoleces were measured and observed. Results After implantation of irradiated echinococcus protoscoleces in mice, the incidence of echinococcosis was 100% in the control group, 80.0% in the 10 Gy group, 33.3% in the 20 Gy group, 33.3% in the 40 Gy group, and 26.7% in the 80 Gy group. The echinococci had lower weight in the irradiated groups with 10 Gy（35.80 mg）, with 20 Gy（0.00 mg）, with 40 Gy（0.00 mg）, and with 80 Gy（0.00 mg） as compared with the control group（157.80 mg）. The echinococcus generated from the protoscoleces appeared calcification. Conclusions γ-ray irradiation could inhibit the formation of echinococcus granulosus generated from protoscoleces, damage the structure of echinococcus generated from the protoscoleces. Irradiation might prevent echinococcosis recurrence after surgical removal of hydatid cysts.

关 键 词：γ-射线 细粒棘球蚴原头节 放射治疗 生长抑制 

分 类 号：R657.3[医药卫生—外科学]