五鹤续断总皂苷对AD大鼠学习记忆及海马Ach代谢的影响  

Effect of Dipsacus total saponins on the ability of learning and memory and acetylcholine metabolism of hippocampus in AD rats

作　　者：万秋英[1] 

机构地区：[1]湖北民族学院附属民大医院,恩施445000

出　　处：《中国应用生理学杂志》2015年第1期82-84,共3页Chinese Journal of Applied Physiology

摘　　要：目的:研究五鹤续断总皂苷对AD大鼠学习记忆能力的影响及其作用机制。方法:将40只大鼠随机分为空白对照组、模型组、五鹤续断组和阳性对照组(n=10),观察各组大鼠一般情况,以方形水迷宫评价大鼠的学习记忆能力,采用双抗体夹心法测定大鼠海马区乙酰胆碱酯酶(Ach E)和胆碱乙酰转移酶(Ch AT)的活性变化。结果:治疗过程中,模型组大鼠一般情况无明显变化,五鹤续断组和阳性对照组大鼠一般情况逐步改善,活动能力逐渐增强。与空白对照组比,模型组不同时间的游泳时间均明显延长,错误次数显著增多,海马区Ach E活性明显增强,Ch AT活性显著降低(P<0.01);与模型组比,五鹤续断组和阳性对照组大鼠不同时间的游泳时间明显缩短,错误次数显著减少,海马区Ach E活性明显降低,Ch AT活性显著增强(P<0.01);与阳性对照组比,五鹤续断组不同时间的游泳时间、错误次数和大鼠海马区Ach E和Ch AT活性无显著性差异。结论:五鹤续断总皂苷能够改善AD大鼠学习记忆能力,其作用机制可能与其调节海马区Ach代谢有关。Objective： To study the effects of Dipsacus total saponins on the ability of learning and memory and its mechanism of action. Methods： Forty rats were randomly divided into blank control group,model group,Dipsacus group and positive control group（ n = 10）,general situation of rats were observed,the ability of learning and memory of rats was tested by Square water maze,the activities of acetylcholinesterase（ ACh E） and choline acetyltransferase（ Ch AT） of hippocampus in rats were measured using double antibody sandwich method. Results： During the period of treatment,general situation had no obvious change in model group,but general situation and the ability of activity were gradually improved in Dipsacus group and positive control group. Compared with blank control group,the swimming time was obviously prolonged and the number of mistakes was obviously increased at different time,the activity of ACh E was significantly enhanced and the activity of Ch AT was significantly decreased in model group. Compared with model group,the swimming time was obviously shortened and the number of mistakes was obviously reduced at different time,the activities of ACh E were significantly decreased and the activities of Ch AT were significantly enhanced in Dipsacus group and positive control group; Compared with positive control group,the swimming time and the number of mistakes at different time and the activities of ACh E and Ch AT had no significant difference in Dipsacus group. Conclusion： Dipsacus total saponins can improve the ability of learning and memory in Alzheimer＇s disease（ AD） rats,its mechanism of action may be related to regulating ACh metabolism of hippocampus.

关 键 词：五鹤续断总皂苷 阿尔茨海默病 海马 乙酰胆碱酯酶 胆碱乙酰转移酶 

分 类 号：R285.5[医药卫生—中药学]