组织工程骨血管生成的研究进展  

RESEARCH PRO GRESS OF ANGIO GENESIS IN VASCUL ARIZED TISSUE ENGINEERED BONE

作　　者：段鑫[1] 李伟[1] 项舟[1] 

机构地区：[1]四川大学华西医院骨科,成都610041

出　　处：《中国修复重建外科杂志》2015年第2期239-244,共6页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家自然科学基金资助项目(31370984)~~

摘　　要：目的对组织工程骨血管生成过程中种子细胞及相关细胞因子研究作一综述。方法广泛查阅近年来组织工程骨血管生成相关文献,对常见种子细胞来源、生物学特性、转变机制、相关细胞因子及信号通路等进行综述。结果随着显微外科技术、基因工程技术的发展,组织工程骨血管化细胞共培养体系的研究已有长足进展,无论是诱导性多能干细胞的引入,还是VEGF和血管生成素1双转染MSCs-内皮祖细胞共培养体系,均在骨生成和血管生成中显示出明显优势,但距临床应用仍有距离。结论采用基因修饰构建的种子细胞-细胞因子-支架材料复合物具有血管生成速度快、效率高、稳定性好的优势,在组织工程骨血管化中具有良好应用前景。Objective To review the research progress of the role of seed cells and related cytokines in angiogenesis of the vascularized tissue engineered bone. Methods The latest literature of tissue engineered bone angiogenesis was reviewed, including the common source of seed cells, biological characteristics, transformation mechanism, related cytokines, and signaling pathways in re-vascularization. Results Microsurgery technique, genetic technique, and co-culture system of vascularized tissue engineered bone have developed to a new level. Moreover, both the induction of introduced pluripotent stem cells and vascular endothelial growth factor-angiopoietins 1 transfected mesenchymal stem cells and endothelial progenitor cells have some advantages for bone regeneration and vascularization. However, all the techniques were not used in clinical practice. Conclusion Using techniques of genetically modified seed cells, related cytokines, and scaffolds may have bright prospects for building vascularized tissue engineered bone.

关 键 词：组织工程骨 血管生成 种子细胞 细胞因子 

分 类 号：R318.11[医药卫生—生物医学工程]