不同时间的UV-B辐射对拟南芥幼苗生长的影响  

Effect of Different Times of UV-B Radiation on Seedling Growth of Arabidopsis thaliana

作　　者：韩雯[1] 韩榕[1] 

机构地区：[1]山西师范大学生命科学学院,临汾041004

出　　处：《植物学报》2015年第1期40-46,共7页Chinese Bulletin of Botany

基　　金：国家自然科学基金(No.30671061);山西省自然科学基金(No.20081101)

摘　　要：以哥伦比亚野生型拟南芥(Arabidopsis thaliana)为实验材料,用辐射功率为16.67μW·cm–2但不同时间(0.5、1、1.5、2、2.5和3小时)的UV-B辐射对拟南芥幼苗进行处理,观察叶片形态,并测定其根长、叶绿素含量、可溶性蛋白含量、丙二醛(MDA)浓度、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性及叶绿素荧光参数。结果显示,短时间UV-B辐射可促进拟南芥根的伸长,叶绿素和可溶性蛋白含量升高;长时间UV-B辐射则抑制拟南芥根的生长,使叶绿素含量、可溶性蛋白含量、叶绿素荧光参数Fv/Fm及q P逐渐降低,MDA浓度、SOD活性、CAT活性和q N值升高,并随着时间的延长逐渐降低或升高。当辐射功率为16.67μW·cm–2时,其最佳辐射时间为1.5小时。UV-B辐射作为一种环境胁迫,其胁迫程度都是在一定的范围内,当胁迫达到极限时,植株都会对UV-B辐射产生一定的适应效应而使损伤降低。We treated Columbia-0 wild-type Arabidopsis thaliana seedlings with enhanced UV-B radiation （16.67 IJW.cm-2 radiant power） at 0.5, 1, 1.5, 2, 2.5, and 3 h. We observed leaf morphological characters and detected root length, leaf chlorophyll content, soluble protein content, malondialdehyde （MDA） concentration, superoxide dismutase （SOD） activity, catalase （CAT） activity and chlorophyll fluorescence characteristics. With a short period of UV-B radiation, roots were elongated and chlorophyll content and soluble protein content were increased in leaf, but prolonged UV-B radiation inhibited the elongated root length; caused decreased leaf chlorophyll content, soluble protein content and chlorophyll fluorescence characteristics such as FJFm and qP; promoted MDA concentration; and increased SOD and CAT activity and qN over time. With 16.67 pW-cm-2 radiation, the optimal time of UV-B radiation was 1.5 h. If UV-B ra- diation acts as a kind of environmental stress, the extent of damage to the plant was restricted, and the plant would have to adapt to UV-B radiation to let the damage reach a minimum when the stress level reached the limitation.

关 键 词：拟南芥 UV-B辐射 幼苗生长 

分 类 号：Q945.78[生物学—植物学]