胰高血糖素样肽1或其类似物体外生物活性检测方法的建立  

Establish the Method for the Detection of GLP-1 or GLP-1 Analogue Biological Activity in vitro

作　　者：邵妤[1,2] 赵威 王微[1,4] 曲国龙[1] 阚乃鹏 凌婧怡[1] 谭俊杰[1] 刘刚[1] 陈惠鹏[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京100071 [2]安徽大学生命科学学院,安徽合肥230601 [3]长春西诺生物科技有限公司,吉林长春130012 [4]吉林大学生命科学学院,吉林长春130012

出　　处：《生物技术通讯》2015年第1期98-101,共4页Letters in Biotechnology

基　　金："十二五"重大新药创制国家科技重大专项"综合性新药研发技术大平台"(2012ZX09301003)

摘　　要：目的:通过对不同活性检测方法的综合比较,筛选最适的胰高血糖素样肽1(GLP-1)或其类似物的体外活性检测方法,为GLP-1类似物的体外生物活性检测奠定基础。方法:以GLP-1作为阳性药物,用MTT方法检测其对RIN-m-5F、MIN6细胞增殖的影响;用ELISA方法检测其对INS-1、MIN6细胞胰岛素分泌量的影响;用ELISA方法检测其对BHK-GLP-1R细胞cAMP分泌水平的影响。通过对上述方法的综合比较,筛选出最适的活性检测方法。结果:GLP-1对细胞增殖的影响实验结果并不显著,对胰岛素分泌量影响的实验效果明显,对cAMP分泌水平的实验无明显效果。结论:ELISA检测GLP-1或其类似物对MIN6细胞胰岛素分泌量实验可用于GLP-1或其类似物的体外活性检测。Objective： Through a comprehensive comparison of different kinds of active detection methods, in order to screening the most suitable bioactivity detection method for the detection of glucagon-like polypetide 1 （GLP-1） or GLP-1 analogue biological activity in vitro. Methods： GLP-1 protein was used as the positive drug to test its effect on the proliferation of RIN-m-5F and MIN6 cells by MTT method, on the amount of insulin secreted by INS-1 and MIN6 cells, and on the level of cAMP secreted by BHK-GLP-1R cells by ELISA method. After a comprehensive comparison of the above methods, screening out the optimal activity detection method. Results： The effect of GLP-1 on cell proliferation was not significant, on the insulin secretion achieved good results, on the level of cAMP secretion experiments no significant effect. Conclusion： The method for the detection of GLP-1 or GLP-1 analogue biological activity in vitro was established, in which ELISA was applied to test the ef- fect of GLP-1 or GLP-1 analogue on the amount of insulin secreted by MIN6 ceils.

关 键 词：2型糖尿病 胰高血糖素样肽1 类似物 活性检测 

分 类 号：Q502[生物学—生物化学]