实时荧光定量PCR相对定量法检测循环miRNA的内参选择  

Selection of Endogenous Reference Gene for RT-qPCR Analysis of Circulating microRNA Levels

作　　者：李雅楠[1] 郝玉宾[2] 张志[3] 戴二黑[3] 

机构地区：[1]河北医科大学,河北石家庄050017 [2]河北省人民医院,河北石家庄050051 [3]河北省石家庄市第五医院,河北石家庄050021

出　　处：《生物技术通讯》2015年第1期146-148,共3页Letters in Biotechnology

基　　金：国家"十二五"科技重大专项(2013ZX10003002-004);河北省卫生厅科研基金(20130650)

摘　　要：microRNA(miRNA)是一类内源性非编码微小RNA,在调控细胞增殖、分化、凋亡等方面发挥重要作用。研究发现,miRNA能够稳定存在于细胞外液,被称为循环miRNA。在多种疾病状态下循环miRNA表达谱改变,具有成为非创伤性新型生物标志物的潜能。实时荧光定量PCR(RT-qPCR)是检测miRNA表达变化的确证实验,其中内参的选择关系到相对定量结果是否真实可靠。我们简要综述国内外实验室常用的RT-qPCR内参及其特点。microRNA（miRNA） are a major class of small endogenous noncoding RNA molecule that participate many biological processes, such as cell proliferation, differentiation and apoptosis. Recent studies indicate that miRNA can stably exist in extraeellular fluid, that is circulating miRNA. The expression profiles of circulating miRNA change under the condition of diseases and it makes circulating miRNA a new noninvasive biomarker for disease. The gold standard methodology for quantifying miRNA expression profiles is RT-qPCR and a suitable endogenous reference gene is of great importance for accurate results. This article will talk about the endogenous reference genes in use and their characteristics.

关 键 词：循环miRNA 实时荧光定量PCR 内参 

分 类 号：Q78[生物学—分子生物学]