EV71及CA16不同检测方法敏感度及特异性比较  

Evaluate the Sensitivity and Specificity of Differentiating Enterovirus 71 and Coxsackievirus A16

作　　者：李华[1] 杨婷[1] 姜广菊 何晓娟[1] 岳磊[1] 谢天宏[1] 杨水芝[1] 朱凡丽 龙润乡[1] 杨蓉[1] 罗芳宇 谢忠平[1] 

机构地区：[1]中国医学科学院/北京协和医学院医学生物学研究所,昆明650118

出　　处：《医学研究杂志》2015年第1期58-62,共5页Journal of Medical Research

基　　金：云南省重点新产品研究项目(2012BC006)

摘　　要：目的比较检测肠道病毒71型(enterovirus 71,EV71)及柯萨奇病毒A组16型(coxsackievirus A16,CA16)不同检测方法、不同生产厂家(A、B、C、D、E)荧光定量PCR检测试剂的敏感度及特异性。方法对EV71及CA16各10个病毒株进行稀释,取原倍、10-3及10-63个浓度,分别采用实时荧光定量PCR法(分别用不同厂家的检测试剂)、RT-PCR法、ELISA法及细胞培养4种方法进行检测,每种检测连续重复3次,然后将其结果进行比较分析。结果 EV71及CA16经不同方法及试剂检测后结果显示,荧光定量PCR方法敏感度最高,依次为细胞培养法、RT-PCR(VP1)法及ELISA法;细胞培养法、RT-PCR(VP1)法及ELISA法的特异性较荧光定量PCR检测方法的特异性高,但荧光定量PCR检测试剂均出现不同程度非特异的假阳性结果;不同的荧光定量PCR检测试剂中,D厂家试剂检测EV71和C厂家试剂检测CA16的敏感度和特异性最高。结论 RT-PCR(VP1)方法更适于检测EV71及CA16,如条件许可则建议联合采用细胞培养检测方法,提高检测结果的敏感度和特异性。Objective To evaluate the sensitivity and specificity of detecting EV71 and CA16 with four different methods and manu- facturers（ A, B,C,I）, E）of the real -time PCR. Methods Ten EV71 strains and ten CA16 strains were diluted to three different concentrations, respectively,the original,10^-3 and 10^-6 times,which were detected simultaneously by the real- time PCR （kits from five manufacturers） ,RT - PCR,ELISA and Cell culture. Each test repeated three times, and then we compared the results. Results real - time PCR got highest sensitivity, followed by Cell culture, RT - PCR and ELISA. The specificity of cell culture, RT - PCR and ELISA were higher than the real -time PCR, but there were non - specificity and false positive result by the method of the real - time PCR. EVT1 from D manufacturer achieved the highest sensitivity and specificity. CA16 from C manufacturer achieved the highest sensitivity and speci- ficity. Conclusion RT - PCR （VP1） is more suitable for detecting EV71 and CA16. It suggests that combining cell culture will improve sensitivity and specificity if the condition allows.

关 键 词：手足口病 EV71 CA16 敏感度 特异性 

分 类 号：R446.1[医药卫生—诊断学]