水通道蛋白1在不同转运功能腹膜组织表达及意义  

Expression of aquaporin-1 in human peritoneum and its effect on peritoneal dialysis patients with the different peritoneal transport characteristics

作　　者：周君[1] 陈菊[1] 

机构地区：[1]中南大学湘雅医学院附属海口医院肾内科,海口570208

出　　处：《中国医师杂志》2015年第1期33-36,共4页Journal of Chinese Physician

基　　金：海南省卫生厅资助项目（琼卫2012LX-15）

摘　　要：目的探讨水通道蛋白1（AQP1）在不同转运功能腹膜透析（PD）患者腹膜组织的表达及意义。方法非慢性肾脏病的腹部择期手术患者为正常对照组；慢性肾脏病5期进行PD置管的30例患者为研究组，为预防大网膜包裹常规切除部分大网膜，采用Western blot和免疫组化方法检测AQP1在腹膜组织中表达，同时置管术后4周进行标准腹膜平衡试验判断腹膜转运功能。结果Western blot结果显示腹膜组织表达28kDa的AQP1蛋白，正常对照组相对表达强度1．49±0．67，研究组为1．61±0．76，两组比较差异无统计学意义（P〉0．05）；而研究组中各组患者比较差异有统计学意义（P〈0．05）。免疫组化显示腹膜毛细血管和小静脉内皮细胞、间皮细胞均可见AQP1蛋白表达，正常对照组内皮细胞AQPl分数2．12±0．18，研究组为2．50±0．81，两组比较差异无统计学意义（P〉0．05）；正常对照组间皮细胞AQP1分数1．95±0．67，研究组为2．23±1．07，两组比较差异无统计学意义（P〉0．05），且内皮细胞和间皮细胞AQP1表达差异无统计学意义（P〉0．05），而在研究组各组间比较差异有统计学意义（P〈0．05）。结论AQP1在不同腹膜转运功能的PD患者表达程度存在差异，其中高转运组和高平均转运组AQP1蛋白表达明显降低，而低转运组明显增加，可解释高转运和高平均转运水转运差，低转运患者水转运好，提示AQP1在腹膜水转运起了重要作用。Objective To investigate the expression of aquaporin-1 （ AQP1 ） in human peritoneum and its effect on peritoneal dialysis （PD） patients with the different peritoneal transport characteristics. Methods Peritoneal biopsies were obtained from the PD patients （ n = 30） at catheter insertion. Western blot and immunohistochemical staining were used to investigate AQP1 expression in peritoneal tissues. After catheter insertion, 4 week, peritoneal equilibrium test （PET） was used to decide peritoneal transport char-acteristics. Results Western blot revealed a major band at 28 kDa band. The expression of AQP1 index was 1.49±0. 67 in the normal subjects, and 1.61±0. 76 in the PD patients, and there was no significant difference. Positive deposition was distributed in mesothelial cells, endothelial cells of capillaries, and small veins. The index of AQP1 expression in endothelial cells of capillaries in normal subjects and PD patients were 2. 12 _＋ 0. 18, and 2. 50±0. 81, respectively, and no significantdifference was found. The index of AQP1 expression in mesothelial cells of capillaries in normal subjects and PD patients were 1.95±0. 67, and 2. 23±1.07, respectively, and no significant difference was found. Among PD patients, the level of AQP1 was significantly different. Conclusions The expression of AQP1 was different in the PD patients with different peritoneal transport characteristics. In the high and high average transport groups, the level of AQP1 was lower; the low transport group was higher. It reveals that AQP1 plays a role in the pathogenesis of transcellular water transport.

关 键 词：水孔蛋白质类/生物合成 腹膜透析 腹膜/代谢 免疫组织化学 印迹法 蛋白质 

分 类 号：R459.5[医药卫生—治疗学]