氟西汀对抑郁大鼠模型海马CA1区、CA3区及齿状回区神经生长因子表达的影响  

Effect of Fluoxetine on Nerve Growth Factor Expression in Hippocampus CA1,CA3 and DG of Rat Depression Model

作　　者：宋晓灵[1] 张芳[1] 曹莉莎[1] 王继生[1] 

机构地区：[1]四川省绵阳市第三人民医院,四川绵阳621000

出　　处：《武汉大学学报（医学版）》2015年第2期185-188,共4页Medical Journal of Wuhan University

基　　金：四川省卫生厅科研课题(编号:120320)

摘　　要：目的:建立慢性温和不可预见性应激(CUMS)大鼠抑郁模型,研究氟西汀对CUMS大鼠海马CA1区、CA3区及齿状回区神经生长因子(NGF)表达的影响。方法:采用CUMS方法建立抑郁大鼠模型。氟西汀(3mg/kg)在造模完成后灌胃给药,每天一次,连续21d。旷场实验、强迫游泳实验检测大鼠行为学变化;免疫组化检测海马CA1、CA3及齿状回NGF的分布与表达。结果:与正常组相比,模型组大鼠水平运动和垂直运动减少(P<0.01),静止不动的时间较正常组增加(P<0.01)。海马CA1区、CA3区及齿状回区NGF表达强度低,阳性颗粒数目少,且着色浅淡。与模型组比较,氟西汀能明显改善CUMS大鼠的抑郁行为,增高CUMS大鼠海马NGF、细胞阳性表达。结论:抑郁大鼠海马NGF含量的高低与抑郁症的发生发展密切相关,促进海马细胞NGF蛋白的表达,保护神经的可塑性可能是氟西汀抗抑郁的机制之一。Objective：To investigate the effect of fluoxetine on nerve growth factor（NGF）expression in hippocampus CA1,CA3 and DG of chronic unpredictable mild stress（CUMS）induced depression rats.Methods：CUMS was taken to establish rat depression model.Fluoxetine（3 mg/kg·d）was administrated after stress procedure for 21 days.The open-field test and forced swimming test were used to evaluate the depression behaviors of rats.NGF protein expression in hippocampus CA1,CA3 and DG was detected by immunohistochemistry.Results：As compared with that of the control group,administration of stress caused a decrease of the crossing and rearing movement times from open-field test and a significant increase of immobility from forced swimming test.CUMS significantly decreased the expression of NGF protein in rat hippocampus CA1,CA3,and DG.Fluoxetine remarkably improved depressive behavior and significantly increased the expression of NGF protein in CUMS rats.Conclusion：The content of NGF might be closely related with the development of depression in CUMS rats.Protection of neural plasticity may be a novel idea with respect to the mechanism underlying the effect of fluoxetine in the treatment of depression.

关 键 词：氟西汀 抑郁 慢性温和不可预见性应激 神经生长因子 免疫组化 海马 

分 类 号：R961[医药卫生—药理学]