心肌梗死后抑郁大鼠心室肌细胞L型钙电流的动力学改变  

Changes of L-type Calcium Currents of Ventricular Myocytes in Depression Rat After Myocardial Infarction

作　　者：阮兵[1] 梁锦军[1] 石少波[1] 孙丽芳[1] 曲川[1] 王腾[1] 向晋涛[1] 黄鹤[1] 杨波[1] 

机构地区：[1]武汉大学人民医院心内科,湖北武汉430060

出　　处：《武汉大学学报（医学版）》2015年第2期193-198,共6页Medical Journal of Wuhan University

基　　金：国家自然科学基金资助项目(编号:81200139);湖北省自然科学基金资助项目(编号:2014CFB739;2014CFA061)

摘　　要：目的:观察心肌梗死后抑郁大鼠心室肌细胞L型钙电流(ICa-L)的动力学特性。方法:将60只SD大鼠随机分为4组:正常对照组(n=10),心梗组(n=20),抑郁组(n=10),心梗后抑郁组(n=20)。通过结扎冠状动脉前降支建立心肌梗死模型,予慢性不可预见性温和刺激建立抑郁模型,利用酶解法分离梗死周边区心室肌细胞,采用全细胞膜片钳技术记录L型钙电流的变化,研究抑郁对心肌梗死后大鼠心室肌细胞ICa-L的动力学影响。结果:与心梗组比较,心梗后抑郁组的I-V曲线上移,激活曲线右移,并延长失活后恢复时间。结论:抑郁可能通过改变心肌梗死后心室肌细胞L型钙通道的动力学来诱发室性心律失常。Objective：To observe the changes on the activation kinetics of L-type calcium currents of ventricular myocytes in depression rat after myocardial infarction.Methods：Sixty SD rats were randomly divided into four groups：normal control group（n=10）,MI group（n=20）,depression group（n=10）,and post-MI depression group（n=20）.Model（post-MI depression）rats were made by acute ligation of left coronary artery and supplying chronic unpredictable mild stress in order.The whole-cell patch-clamp technique was used to observe the changes on the activation kinetics of L-type calcium currents of actually isolated ventricular myocytes in depression rat after myocardial infarction.Results：Compared with MI group,the I-V curve shifted upwards in postMI depression group,and its steady-state activation curve shifted to the right,and the recovery time from inactivation prolonged.Conclusion：Depression may be induced ventricular arrhythmias by changing the activation kinetics of L-type calcium currents of ventricular myocytes in depression rat after myocardial infarction.

关 键 词：心肌梗死 抑郁 心室肌细胞 L型钙电流 膜片钳技术 

分 类 号：R542.22[医药卫生—心血管疾病]