黄芪多糖对严重烫伤大鼠肠道免疫功能的影响  

Effects of astragalus polysaccharide on intestinal immune function of rats with severe scald injury

作　　者：黄翠兰[2] 詹剑华[1] 罗锦花[1] 

机构地区：[1]南昌大学第一附属医院烧伤科,330006 [2]江西省人民医院重症医学科,330006

出　　处：《中华烧伤杂志》2015年第1期30-36,共7页Chinese Journal of Burns

基　　金：江西省科技计划（ 20142 BBG70065 ）

摘　　要：目的 观察黄芪多糖对烫伤大鼠肠黏膜形态、肠黏液分泌型IgA（s-IgA）水平、派伊尔结T淋巴细胞亚群分布的影响. 方法 取130只成年SD大鼠,按随机数字表法分为假伤组10只（模拟致伤）、烫伤组30只、低剂量组30只、中剂量组30只和高剂量组30只,后4组大鼠背部造成30％ TBSAⅢ度烫伤.伤后2h起,低、中、高剂量组大鼠每天腹腔注射0.5 mL黄芪多糖,剂量分别为100、200、300 mg/kg;烫伤组大鼠注射0.5 mL生理盐水.假伤组于伤后即刻,其余4组均分别于伤后3、7、14 d,每组取10只大鼠,HE染色观察回肠黏膜形态,双抗体夹心ELISA法测定肠黏液s-IgA水平,流式细胞仪检测小肠派伊尔结CD3＋、CD4＋、CD8＋T淋巴细胞百分比并计算CD4＋/CD8＋比值.对数据行单因素方差分析、析因设计方差分析、SNK检验. 结果 （1）假伤组大鼠伤后即刻肠黏膜绒毛形态正常,绒毛上皮细胞完整.各烫伤组大鼠伤后3d肠黏膜绒毛水肿,绒毛上皮细胞大片坏死、脱落,大量炎性细胞浸润;伤后7d,该4组大鼠肠黏膜病变情况与伤后3d相近;伤后14d,烫伤组大鼠肠黏膜病变仍未见明显改善,低、中剂量组大鼠肠黏膜病变明显减轻,高剂量组大鼠肠黏膜形态恢复至与假伤组接近.（2）与假伤组伤后即刻[（69 ±4） μg/mL]比较,烫伤组及低、中、高剂量组大鼠伤后3、7、14 d s-IgA水平[（43±5）、（45±5）、（46±5）μg/mL,（47 ±5）、（48±5）、（49 ±6） μg/mL,（50±6）、（51±5）、（52±5）μg/mL,（53±6）、（54±5）、（55±5）μg/mL]显著降低（P值均小于0.05）.中剂量组大鼠伤后各时相点s-IgA水平显著高于烫伤组（P值均小于0.05）,高剂量组大鼠伤后各时相点s-IgA水平显著高于烫伤组与低剂量组（P值均小于0.05）.（3）与假伤组伤后即刻比较,除高剂量组大鼠伤后14 d外,各烫伤组大鼠伤后各时相点CD3＋、CD4＋T淋巴细胞百分比显著降低（P值均小于Objective To observe the effects of astragalus polysaccharide （AP） on the intestinal mucosal morphology,level of secretory IgA （s-IgA） in intestinal mucus,and distribution of T lymphocyte subsets in Peyer＆#39;s patch in rats with severe scald injury.Methods One hundred and thirty SD rats were divided into sham injury group （SI,sham injured,n =10）,scald group （S,n =30）,low dosage group （LD,n =30）,moderate dosage group （MD,n =30）,and high dosage group （HD,n =30） according to the random number table.Rats in the latter 4 groups were inflicted with 30% TBSA full-thickness scald on the back.From post injury hour 2,rats in groups LD,MD,and HD were intraperitoneally injected with 0.5 mL AP solution with the dosage of 100,200,and 300 mg/kg each day respectively,and rats in group S were injected with 0.5 mL normal saline instead.Ten rats from group SI immediately after injury and 10 rats from each of the latter 4 groups on post injury day （PID） 3,7,14 were sacrificed,and their intestines were harvested.The morphology of ileal mucosa was examined after HE staining; the level of s-IgA in ileal mucus was determined with double-antibody sandwich ELISA method; the proportions of CD3 ＋,CD4-,CD8 ＋ T lymphocytes in Peyer＆#39;s patches of intestine were determined with flow cytometer,and the proportion of CD4 ＋ to CD8 ＋ was calculated.Data were processed with one-way analysis of variance,analysis of variance of factorial design,and SNK test.Results （1） Villi in normal form and intact villus epithelial cells were observed in rats of group SI immediately after injury,while edema of villi and necrosis and desquamation of an enormous amount of villi were observed in groups with scalded rats on PID 3,with significant infiltration of inflammatory cells.On PID 7,no obvious improvement in intestinal mucosal lesion was observed in groups with scalded rats.On PID 14,the pathology in intestinal mucosa of rats remained nearly the same in group S,and it was alleviated obviously in groups LD and MD

关 键 词：烧伤 肠 免疫 T淋巴细胞亚群 免疫球蛋白A 分泌 黄芪多糖 

分 类 号：R285.5[医药卫生—中药学]