p53基因状态对体外微核试验结果影响的研究进展  

Impact of p53 gene status on in vitro micronucleus test:research progress

作　　者：欧红梅[1] 周长慧[1] 涂宏刚[1] 常艳[1] 

机构地区：[1]中国医药工业研究总院上海医药工业研究院国家上海新药安全评价研究中心,上海201203

出　　处：《中国药理学与毒理学杂志》2015年第1期170-173,共4页Chinese Journal of Pharmacology and Toxicology

基　　金：国家科技重大专项(2012ZX09302002)~~

摘　　要：近年来,体外哺乳动物细胞遗传毒性试验假阳性率高的不足引起了毒理学研究者的关注。体外微核试验常用的啮齿类动物细胞系如CHO,V79,CHL和L5178Y,易产生假阳性结果,可能原因之一是啮齿类动物细胞系的p53基因为突变型,即p53基因功能缺陷。但也有观点认为,p53基因功能不同的细胞系不影响体外微核试验结果的判定。本文主要比较了不同细胞系体外微核试验结果,包括常用的p53基因功能缺陷的啮齿类动物细胞系、p53基因功能完整的细胞系和p53基因功能缺失的NH32细胞。希望有助于阐明遗传毒性化合物引起p53功能不同的细胞系的DNA损伤的机制,合理选择体外微核试验系统,从而降低体外微核试验结果的假阳性率。In recent years,it has beocme a major concern that mammalian cell tests for genotoxicity can cause a high rate of false positive results. The rodent cell lines（ V79,CHL,CHO,and L5178Y）cause a higher rate of false positive results than human primary lymphocytes and TK6 cells. One possible reason is that the p53 gene of rodent cell lines is mutated. However,these is also the view that p53 gene status in different cells does not affect the results of in vitro micronucleus assay. In this paper,we mainly compare the result of in vitro micronucleus test on different cell lines,including p53-compromised rodent cell lines,p53-competent human lymphocytes cells and p53-abrogated NH32 cells. This paper is expected to help explain the mechanism of DNA damage in various p53 gene function cells,select the optimal test systems and reduce the frequency of false positive reactions in the long run.

关 键 词：P53基因 体外微核试验 假阳性反应 

分 类 号：R114[医药卫生—卫生毒理学]