热缺血时间对无心跳大鼠胰岛获取和功能的影响  

Effect of warm ischemia time to the islet function on the non-heart-beating donor rat

作　　者：宋文卿[1] 刘永锋[2] 程颖[2] 车彦鹏[1] 梁世博[1] 

机构地区：[1]大庆龙南医院(齐齐哈尔医学院第五附属医院)普外科,黑龙江省大庆市163453 [2]中国医科大学附属第一医院肝胆外科暨器官移植科

出　　处：《中华器官移植杂志》2015年第1期40-43,共4页Chinese Journal of Organ Transplantation

基　　金：黑龙江省卫生厅科研课题（2012-497）

摘　　要：目的观察不同热缺血时间对获取的大鼠胰岛及胰岛功能的影响。方法采用雄性Wistar大鼠。在大鼠心跳停搏后，获取热缺血时间分别为0min、15min、30min、45min的4组大鼠胰腺，给予4℃的UW液保存8h，再将胶原酶溶液10ml注入胰腺。胰岛获取后计算各组胰岛的纯度、活率及活性，并进行统计分析。结果0rain组、15rain组、30min组和45rain组获取的胰岛数分别为（433±41）、（396±38）、（350±31）和（66±17）IEQ/只，胰岛活率分别为94％、88％、77％和25％，胰岛纯度分别为88％、78％、60％和32％，胰岛素释放指数分别为2．38±0．23、2．25±0．18、2．19±0．18和1．25±0．12。与0min组相比，15rain组和30min组获得的胰岛数量、纯度、活率及活性的差异均无统计学意义（P〉0．05），45min组获得的胰岛数量、纯度、活率及活性与0min组相比，差异有统计学意义（P〈0．05），低于临床上的胰岛移植标准（活性〉75％，纯度〉50％）。结论热缺血时间在30min以内对无心跳大鼠胰岛无明显影响，可用于胰岛移植。热缺血时间达45min时，对大鼠胰岛可产生明显影响，不宜用于胰岛移植。Objective To observe the influence of warm ischemia time on acquisition of rat pancreatic islets and islet function. Method Male Wistar rats were used. After heart beats stopped, the pancreases in four groups of rats were harvested, and warm ischemia time was 0, 15, 30 and 45 min separately. The pancrease was preserved in UW at 4℃ for 8 h, and subjected to injection of collagenase solutions. After islets were acquired, the purity, survival rate and islet activity were tested, and statistical analysis was performed. Result The number of islets obtained in 0 rain group, 15 min group, 30 min group and 45 rain group was （433±41）, （396±38）, （350±31） and （66±17） IEQ/one, islet viability was 94%, 88%, 77% and 25%, and purity was 88%, 78%, 60% and 32%, and insulin release index was 2. 38 ± 0. 23, 2. 25 ± 0. 18,2. 19 ± 0. 18 and 1.25±0. 12, respectively. There was no significant difference in islet number, purity, survival rate and activity 15 min group and 30 min group between 15 min group or 30 min group and 0 min group （P〉0. 05）. There was significant difference between 45 min group and 0 min group in islet number, purity, survival rate and activity （P〈0. 05 ）. The survival rate and purity in 45 min group were lower than the clinical standards for islet transplantation （survival rate 〉 75%, and purity 〉 50%）. Conclusion Warm ischemia time of 15 rain in non-heart-beating brain death（NHBD） rats had no effect on islet isolation and purification. Warm ischemia time within 30 rain showed no significant influence on islets of NHBD rats, which can be used in islet transplantation. influence on islets of NHBD rats, which can＇t be Warm ischemia time at 45 min showed significant used in islet transplantation.

关 键 词：大鼠 胰岛 热缺血时间 

分 类 号：R587[医药卫生—内分泌]