最大摄氧量标志的有氧能力可训练性相关的尿游离miRNA表达谱特征分析  

Diagnostic Pattern of Urine Cell-free MicroRNA Related to Trainability of Aerobic Athletic Ability Indicated by Maximal Oxygen Uptake

作　　者：薛源[1] 李岩[2] 徐思[3] 

机构地区：[1]四川师范大学体育学院,四川成都610101 [2]鲁东大学体育学院,山东烟台264025 [3]四川省医学科学院,四川成都610031

出　　处：《武汉体育学院学报》2015年第3期84-90,共7页Journal of Wuhan Sports University

基　　金：国家自然科学基金研究项目(81173281);国家"863"计划研究项目(2008AA02Z445);四川省教育厅研究项目(11SA055)

摘　　要：目的:分析有氧训练全过程尿游离miRNA(UCF-miRNA)表达谱特征与有氧运动能力可训练性水平的关系,探讨其作为生物标记预测和评估有氧耐力的可训练性的可行性并构建关联模型用于运动员选材和训练。方法:采用关联研究法,研究92名男性青少年运动员15周有氧训练前后最大摄氧量相对值(R-VO2max)变化率与单次有氧训练UCF-miRNAs表达模式特征的关联性。结果:16个在功能上与运动性适应调控相关的UCF-miRNAs构成的特异性表达差异谱与显著高于平均水平的RVO2max提高率表型有关联,其模式特征与良性运动性适应的生理反应特征一致。结论:运动性适应UCF-miRNAs特征性表达谱模式与有氧运动能力的可训练性存在关联并反映其适应生理特点及状态,可用来早期预测有氧运动能力发展潜力、评价训练效果、竞技状态和早期诊断运动伤病。The objective was to analysis the correlation between the expression profile of Urine cell-free miRNAs （UCF- miRNAs） and the trainability of athletic ability, and to build the model of UCF-miRNAs expressing pattern reflecting the trainability of aerobic athletic ability. 92 male athletes were engaged in a 15-week aerobic training, and the maximal oxy- gen uptake （VO2max） before and after the training were measured, and then correlated to the characters of UCF-miR- NAs＇ Differential Expression Profile which extracted and analyzed stimutaneously. 16 UCF-miRNAs, which were known as closely related to exercise adoption, and their expressing pattern were extracted, validated, and confirmed to constitute the differential expression profile of HR group is characteristic of higher growth ratio of VO2max, and whose Differential Expression Profile coincided with the positive characters of exercise adaption. UCF-miRNAs＇ Differential Expression Pro- file was closely related to the trainability of aerobic athletic ability, which could be used as a method to forecast the poten- tial of aerobic athletic ability, to value the effect of training, and to diagnose sport hurts, early and accurately.

关 键 词：最大摄氧量 有氧运动能力 尿游离-miRNA 可训练性 关联模型 运动员选材 

分 类 号：G804.21[文化科学—运动人体科学]