四溴双酚A对HepG2细胞线粒体膜电位及凋亡的影响  

Effect of Tetrabromobisphenol A on Mitochondrial Membrane Potential and Apoptosis of HepG2 Cells

作　　者：向明灯[1] 李良忠[1] 玉琳[1] 于云江[1] 

机构地区：[1]环境保护部华南环境科学研究所环境健康研究中心

出　　处：《环境卫生学杂志》2015年第1期6-9,13,共5页JOURNAL OF ENVIRONMENTAL HYGIENE

基　　金：国家自然科学基金项目(21377045)

摘　　要：目的研究不同浓度四溴双酚A(TBBPA)暴露对人肝癌(Hep G2)细胞凋亡的影响。方法以不同浓度TBBPA染毒Hep G2细胞,采用MTS法检测细胞存活率,JC-1荧光探针检测细胞线粒体膜电位变化,以AnnexinⅤ与PI联合标记并使用流式细胞仪检测细胞凋亡。结果 Hep G2细胞存活率随着TBBPA暴露浓度升高相应降低;与对照组相比,20和30μmol/L TBBPA暴露组Hep G2细胞线粒体膜电位显著下降(P<0.01);Hep G2凋亡细胞随TBBPA暴露浓度升高有增加趋势,其中30μmol/L TBBPA暴露组与对照组相比差异显著(P<0.01)。结论 TBBPA可能通过降低线粒体膜电位引起细胞凋亡。Objectives To investigate the effect of tetrabromobisphenol A on the apoptosis of HepG2 cells. Methods The viability of HepG2 cells was measured by MTS assay kit, the effect of tetrabromobisphenol A on mitochondrial membrane potential was measured by using a JC - 1 fluorescence probe ; and the apoptosis of ceils labeled with Annexin Vand PI was detected by flow cytometry. Results The viability of cells was decreased with the increase of TBBPA exposure. Comparing with control cells, the mitoehondrial membrane potential of cells treated with 20 and 30 μmol/L TBBPA was significantly decreased. The apoptosis of cells induced by TBBPA was increased in a dose-dependent manner, and a statistically significant increase of apoptosis was observed at a concentration of 30 μmol/L. Conclusions TBBPA could induce an apoptosis of HepG2 cells, which may be related to the decrease of mitochondrial membrane potential.

关 键 词：四溴双酚A 线粒体膜电位 细胞凋亡 

分 类 号：R114[医药卫生—卫生毒理学]