S-亚硝基谷胱甘肽对腹部创伤大鼠肠黏膜屏障的影响  

Effect of GSNO on intestinal mucosal barrier in rats with abdominal trauma

作　　者：丁凯[1] 虞文魁[1] 冯涛[1] 徐琳[1] 吴波[2] 谌达程[3] 李秋荣[1] 李宁[1] 

机构地区：[1]第二军医大学南京临床医学院(南京军区南京总医院)普通外科,江苏南京210002 [2]第二军医大学南京临床医学院(南京军区南京总医院)病理科,江苏南京210002 [3]第二军医大学南京临床医学院(南京军区南京总医院)肾脏科,江苏南京210002

出　　处：《肠外与肠内营养》2015年第1期42-45,共4页Parenteral & Enteral Nutrition

基　　金：总后司令部重大项目资助(AWS12J001)

摘　　要：目的:探讨S-亚硝基谷胱甘肽(GSNO)对腹部创伤大鼠肠黏膜屏障的保护作用。方法:建立腹部创伤大鼠模型,将24只大鼠随机分为对照组(腹部创伤组)和GSNO组(腹部创伤模型+GSNO干预,50μg/kg),每组12只。分别在第1、6和24 h检测肠黏膜紧密连接蛋白(Occludin、Zo-1、Claudin1/2)的表达水平;FITC-葡聚糖(FD4)对大鼠体内肠黏膜屏障的通透性;肠壁中胶质纤维酸性蛋白(GFAP)阳性细胞的数量。结果:GSNO作用1 h和6 h,血浆中FD4水平显著低于对照组(P<0.05);GSNO处理6 h、24 h,肠黏膜屏障中紧密连接蛋白Claudin、Occludin、Zo-1表达水平均显著高于对照组(P<0.01);GSNO作用6 h和24 h时,GFAP阳性细胞数均显著低于对照组(P<0.01)。结论:使用GSNO干预,可改善腹部创伤大鼠的肠黏膜屏障通透性增加和肠屏障功能损害。Objective： To explore the possible protective effect of GSNO on intestinal mucosal barrier in rats with abdominal trauma. Methods： 24 male SD rats that underwent abdominal trauma and blood loss were randomized into control（ n = 12） or GSNO（ n = 12） groups. Rats in GSNO group received GSNO（ 50 μg / Kg body weight） by intraperitoneal administration. Intestinal barrier integrity（ Occludin、Zo-1、Claudin）,intestinal mucosal barrier permeability（ FD4） and the number of glial fibrillary acid protein（ GFAP） positive cell were observed and detected at the first,sixth and 24 th hour after treatment,respectively. Results： Plasma FD4 level was significantly lower than that in control group in the first and sixth hour（ P〈0. 05）. Expression levels of the tight junction proteins（ Claudin,Occludinn and Zo-1） in the GSNO group were significantly higher than that in the control group in the sixth and24 th hour（ P〈0. 01）. The number of GFAP positive cells in the GSNO group were significantly lower than the control group in the sixth and 24 th hour（ P〈0. 01）. Conclusion： The treatment of GSNO can decrease the intestinal mucosal barrier permeability and decrease the intestinal barrier function injury of the rat model suffering abdominal trauma.

关 键 词：腹部创伤 S-亚硝基谷胱甘肽 肠黏膜屏障完整性 肠胶质细胞 胶质纤维酸性蛋白 

分 类 号：R965[医药卫生—药理学]