两亲性聚乙烯亚胺修饰量子点构建功能性光学探针用于干细胞治疗的示踪研究  

Stem Cell Tracking with Amphiphilic Polyethyleneimine Modified Quantum Dots as Functional Optic Probes

作　　者：温清[1] 刘人恺[1] 王志勇[2] 

机构地区：[1]广东医学院附属南山医院神经内科,深圳518052 [2]中国科学院深圳先进技术研究院,保罗·C·劳特伯生物医学成像研究中心,深圳518055

出　　处：《华中科技大学学报（医学版）》2015年第1期28-31,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：深圳市科技计划资助项目(No.201203213)

摘　　要：目的制备新型分子影像探针标记间充质干细胞,以实现细胞荧光成像。方法应用1-碘十二烷修饰聚乙烯亚胺,构建两亲性大分子。利用大分子自组装性能,包裹疏水性核壳结构CdSe/CdS量子点构建纳米探针。结果该探针在630nm处呈现出较强的荧光信号,能够促使间充质干细胞高效内吞。体内成像结果显示,与空白对照细胞相比,被标记细胞呈现出显著的光学信号,空白组信号值为1.47e8[p/s/cm2/sr]/[μW/cm2],实验组信号值为2.78e8[p/s/cm2/sr]/[μW/cm2]。结论该纳米探针具有良好光学成像性能,可实现细胞标记。Objective Stem cell therapy has been extensively used in clinical practice. However ,the methods available are unable to non‐invasively detect the distribution of cells in vivo. This study was aimed to establish a novel molecular imaging probe for stem cell labeling ,which would monitor cells via fluorescence imaging. Methods 1‐iodododecane was used to modify polyethyleneimine （PEI） to form amphiphilic macromolecules. These macromolecules encapsulated the hydrophobic quantum dots by self‐assembly to form imaging probes. Results Strong fluorescence signals of the probe occurred at 630 nm ,and the probe could be effectively endocyted by mesenchyme stem cells. In vivo imaging showed that significant optic signals occurred in probe‐labeled mesenchyme stem cells compared with the control cells （blank group：1.47e^8 [p/s/cm^2/sr]/[μW/cm^2 ];test group：2.78e^8 [p/s/cm^2/sr]/[μW/cm^2 ]）. Conclusion The nanoprobes have good optic imaging effects ,and can be used to la‐bel cells.

关 键 词：聚乙烯亚胺 量子点 荧光成像 干细胞治疗 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]