微小RNA Let-7/Lin28调节环在鼻咽癌中的表达及意义  

Expression of miRNA Let-7/Lin28 Feedback Loop in Nasopharyngeal Carcinoma and Its Significance

作　　者：黄孝文[1] 饶丽华 许胜[1] 崔伟伟[1] 崔永华[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院耳鼻咽喉头颈外科,武汉430030 [2]湖北省宜昌市第二人民医院耳鼻咽喉头颈外科

出　　处：《华中科技大学学报（医学版）》2015年第1期60-63,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

摘　　要：目的观察miRNA Let-7和Lin28在鼻咽癌组织中的相对表达并分析其相关性,探讨该调节环在鼻咽癌病理机制中的可能作用和意义。方法以正常鼻咽组织为对照,运用实时定量聚合酶链反应(Real-time PCR)方法分别检查鼻咽癌组织中Lin28A/B和Let-7amiRNA的相对表达量,并对两组数据进行相关性分析。采用免疫组织化学技术检查Lin28A/B在鼻咽癌组织中的表达部位和水平。结果 Real-time PCR实验显示,Lin28miRNA在鼻咽癌组织中的相对表达量较对照组升高,而Let-7amiRNA在鼻咽癌组织的相对表达量较对照组降低,差异有统计学意义;鼻咽癌组织中Lin28A/B miRNA和Let-7amiRNA的相对表达量呈负相关。免疫组织化学分析证实,Lin28蛋白在鼻咽癌组织呈阳性表达,定位于细胞质中;而在正常鼻咽组织中,Lin28无表达。结论在鼻咽癌组织中Lin28表达上调而Let-7amiRNA表达下调,且二者呈显著的负相关,提示Let-7/Lin28双向负反馈调节异常可能参与了鼻咽癌的发生发展。Objective To investigate the expressions of miRNA Lin28/Let‐7 ,a bidirectional negative feedback loop ,in na‐sopharyngeal carcinoma（NPC） ,so as to evaluate their role in the pathogenesis of NPC.Methods With normal nasopharyngeal tissues as control ,the expressions of miRNA Lin28A/B and miRNA Let‐7a in NPC were detected respectively by using real‐time PCR technique in NPC tissues and the correlation analysis was performed.Immunohistochemistry（IHC）was used to detect the expression levels and locations of Lin28A/B protein in the NPC tissues. Results Real‐time PCR showed that the expression of miRNA Lin28 was significantly up‐regulated in NPC tissues ,and miRNA Let‐7a was significantly down‐regulated in NPC tis‐sues as compared with the control tissues. There was statistically significant difference in the expressions of miRNA Lin28 and miRNA Let‐7a between the tumor and control tissues. IHC analysis demonstrated positive expression of Lin28 in NPC tissues , which was located in the cytoplasm ,and negative expression of Lin28 in normal nasopharyngeal tissues.Conclusion miRNA Lin28 is up‐regulated and miRNA Let‐7a down‐regulated in NPC ,and there is a significant negative association between them ,which indicates that abnormal bidirectional negative feedback of Lin28/Let‐7 may be involved in the molecular pathogenesis of NPC.

关 键 词：鼻咽癌 Lin28 LET-7 实时定量聚合酶链反应 免疫组织化学 

分 类 号：R766[医药卫生—耳鼻咽喉科]