脊髓损伤后早期小鼠肠道功能的变化  

Intestinal Function Changes in Mice after Spinal Cord Injury in Early Stage

作　　者：梁羽[1] 李灿[1] 罗兰[1] 杨拯[1] 王强[1] 马俊[1] 王洋洋[1] 张璐璐[1] 杨维[1] 易芳[1] 

机构地区：[1]成都医学院,四川成都市610500

出　　处：《中国康复理论与实践》2015年第2期148-152,共5页Chinese Journal of Rehabilitation Theory and Practice

基　　金：国家级大学生创新创业训练计划项目(No.201313705002);成都医学院大学生创新实验计划项目(No.CXXS201307)

摘　　要：目的探讨脊髓损伤后短期内小鼠肠道功能的变化。方法将105只昆明种小鼠随机分为正常组(A组,n=30)、假手术组(B组,n=30)和模型组(C组,n=45)。A组不作处理,B组仅暴露脊髓,不夹持。C组采用动脉瘤夹夹持T10处脊髓,复制脊髓损伤模型。分别于术后12 h、24 h、48 h测定小鼠回肠肌电慢波及平滑肌收缩力,并做回肠HE染色。结果脊髓损伤后12 h、24h、48 h,C组小鼠肌电频率和振幅低于A组和B组(P<0.05),收缩力振幅低于A组和B组(P<0.05),但24 h、48 h后收缩力频率高于A组和B组(P<0.05)。C组各时间点肠黏膜评分均较A组和B组高(P<0.05)。结论小鼠脊髓损伤后早期,肠道平滑肌肌电活动减弱,收缩力减小,肠黏膜轻度损伤。Objective To investigate the influence of spinal cord injury on intestinal function in mice after a short period. Methods 105 Kunming mice were randomly assigned to normal group（group A, n=30）, sham group（group B, n=30） and model group（group C, n=45）.Group A received no treatment, group B was only exposed the spinal cord, while group C was induced by a constant compression with a modified arterial forcep at T10 to establish spinal cord injury model. 12 hours, 24 hours and 48 hours after modeling, myoelectric slow wave activity and tension activity of the ileum were tested, and HE staining was used. Results Compared with groups A and B, the slow wave activity was significantly weaker in group C at every time point（P〈0.05）, so was the amplitude of tension activity（P〈0.05）. Frequency of tension activity was obviously higher in group C than in groups A and B 24 hours and 48 hours after modeling（P〈0.05）. The injury scores were higher in group C than in groups A and B（P〈0.05）. Conclusion There is reduce in myoelectric slow wave activity, tension activity of the ileum and mild injury in intestinal mucosa in mice after spinal cord injury in the early stage.

关 键 词：脊髓损伤 肠道 功能障碍 小鼠 

分 类 号：R651.2[医药卫生—外科学]