补肾通督胶囊对胶原诱导关节炎大鼠下丘脑-垂体-肾上腺轴的影响  

Effect of Bushentongdu Capsule on Hypothalamus- pituitary- adrenal Axis( HPA) of Collagen Induced Arthritis rat

作　　者：赵慧[1] 林琳[2] 朱阳春[2] 侯丽萍[3] 吴建红[1] 

机构地区：[1]湖北中医药大学,湖北武汉430065 [2]北京中医药大学,北京100078 [3]山西省太原市类风湿病医院,山西太原030006

出　　处：《湖北中医药大学学报》2015年第1期13-15,共3页Journal of Hubei University of Chinese Medicine

基　　金：太原市科技项目(12016922)

摘　　要：目的观察补肾通督胶囊对胶原诱导关节炎大鼠下丘脑-垂体-肾上腺轴的影响。方法将大鼠分为正常对照组、模型对照组、补肾通督胶囊低剂量组、中剂量组、高剂量组和雷公藤组6组。第1天在大鼠尾巴基底部的松弛皮肤,皮下注射含有300μgⅡ型胶原蛋白的乳化剂,7d后,皮下再次注射含有100μgⅡ型胶原蛋白的乳化剂,造成胶原诱导关节炎模型。从第13天开始灌胃给药18d,在第31天检测各组大鼠的血清CRH、ACTH和CORT。结果与正常对照组比较,模型对照组大鼠的血清CRH、ACTH和CORT的水平皆有显著降低(P<0.01),补肾通督3组大鼠的血清CRH、ACTH和CORT的水平较模型组皆有显著上升,其中高剂量组的水平与雷公藤组没有显著差异(P>0.05)。结论补肾通督胶囊可以提高大鼠血清CRH、ACTH和CORT的含量,故其临床治疗类风湿性关节炎的作用机制很有可能是由于其对下丘脑-垂体-肾上腺轴的调控作用。Objective To observe the effect of Bushentongdu capsule（ BS） on hypothalamus- pituitary- adrenal axis of collagen induced arthritis rat. Method The rats were randomly assigned to six groups,normal control,model,low dose of BS,medium dose,high dose group and Tripterygium glycosides group. The first day at the end of the loose skin of rats bucky bottom gave emulsifier containing 300μgⅡcollagen in subcutaneous injection,after 7 days,gave subcutaneous injections of collagen- containing emulsifiers 100μgⅡ again,resulting in collagen- induced arthritis model. The rats were administrated with corresponding medicine for 18 days starting from 13 th day. Serum was collected on 31 th day to detect CRH,ACTH and CORT. Results The serum level of CRH,ACTH and CORT were lower in arthritis group than control group（ P 0. 01）. CRH,ACTH and CORT raised in three dose groups. BSH and TG group had no significant difference between high dose group and Tripterygium glycosides group（ P 0. 05）. Conclusion BS increases the level of CRH,ACTH and CORT,its machanism may be related to the modulation of the HPA axis.

关 键 词：胶原诱导关节炎 补肾通督胶囊 下丘脑-垂体-肾上腺轴 

分 类 号：R285.5[医药卫生—中药学]