水稻抽穗期基因Hd6-f1的定位  

Mapping of Hd6-f1 Gene on Rice Heading Date

作　　者：周鹏飞[1] 柳絮[2] 张华[2] 宣宁[2] 李军[2] 杨永义[2] 李广贤[2] 姚方印[2] 刘开启[1] 

机构地区：[1]仲恺农业工程学院,广东广州510225 [2]山东省农业科学院生物技术研究中心,山东济南250100

出　　处：《山东农业科学》2015年第2期12-15,共4页Shandong Agricultural Sciences

基　　金：国家自然科学基金项目(31171529);山东省农业生物资源创新利用课题;山东省农业科学院科技创新重点项目(2014CXZ10-2);山东省现代农业产业技术体系(水稻)项目资助

摘　　要：本试验以明恢63(供体)/早熟豫6(受体)的BC4F2分离群体为材料,采用BSA(Bulked segregation analysis)法,对控制水稻抽穗期分离的基因进行SSR分子标记定位。BC4F2出现抽穗期分离,早抽穗植株为253株,晚抽穗植株为657株,χ2=3.66<P0.05=3.84,符合1∶3的孟德尔分离比,表明F2群体的抽穗期分离受一对等位基因控制,晚抽穗性状为显性。以明恢63×早熟豫6的BC4F2分离群体的253株隐性单株为定位群体,将该抽穗基因(暂时命名为Hd6-f1)定位在水稻第6染色体分子标记RM19771与RM527之间,遗传距离分别为0.2 c M和2.9 c M,与RM19780共分离。Using the BC4F2 segregation populations of Minghui 63 （donor） and Zaoshuyu 6 （receptor） as materials, the SSR markers were located for the gene on rice heading date by the bulked segregation analysis （BSA）. The BC4F2 population had 253 early heading plants and 657 late heading plants（ X2 = 3.66 〈 P0.05 = 3.84 ）. Its segregation accorded with the ratio of 1： 3, which confirmed that the heading date was controlled by a pair of alleles and the late heading character was dominant. Using the 253 early heading plants of BC4F2 population as mapping population, the gene on heading date named Hd6 -f1 was mapped on chromosome 6 between SSR markers RM19771 and RM527 with the genetic distances as 0.2 cM and 2.9 cM respectively, and co -segregated with RM19780.

关 键 词：水稻 抽穗期 BSA SSR分子标记 基因定位 

分 类 号：S511.033[农业科学—作物学]