影响甘蓝小孢子胚状体发生的因素研究  

Study on Factors Influencing Microspore Embryogenesis of Cabbage

作　　者：杨红丽[1] 胡靖锋[1] 徐学忠[1] 和江明[1] 宋爽[2] 

机构地区：[1]云南省农业科学院园艺作物研究所,云南昆明650205 [2]云南省农业科学院行政处,云南昆明650205

出　　处：《山东农业科学》2015年第2期21-24,28,共5页Shandong Agricultural Sciences

基　　金：云南省科技厅重点新产品开发计划(2012BB017);农业部公益性行业专项(201003029);云南省农业厅蔬菜新品种协作项目[云财农(2012)58号]

摘　　要：以6个甘蓝杂交种为试材,采用游离小孢子培养方法,研究影响胚状体发生的因素。结果表明：花蕾长度为3.00～4.00 mm时,有利于获得胚状体;1 500 r/min离心、32.5℃热激24 h,最有利于胚状体的诱导;花蕾经低温4℃预处理24 h,可提高胚诱导率;低速振荡培养能够促进胚状体发生;基因型在小孢子胚状体诱导中起着主导作用。Taking 6 cabbage hybrids as experimental materials, the influence factors of somatic embryo- genesis were studied by isolated microspore culture method. The results showed that the flower bud length in the range of 3.00 ～ 4.00 mm was favorable to obtain embryoid ; when centfifugation at the speed of 1 500 r/min and heat shocking at 32.5℃ for 24 hours, it was the most beneficial to the induction of embryoid; when the flower buds were pretreated at 4℃ for 24 hours, the embryo induction rate could be improved; low oscillation culture could promote the embryogenesis. Genotype played a dominant role in microspore embryoid induction.

关 键 词：甘蓝 小孢子培养 胚状体 

分 类 号：S635.036[农业科学—蔬菜学]