HPLC同时测定了哥王中4种黄酮的含量  

Simultaneous determination of four flavonoids in Wikstroemia indica by HPLC

作　　者：孙丽霞[1,2] 孙立新[1] 慕善学[1] 魏岚[1] 徐翠玲[1] 贾铭婧 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016 [2]沈阳药科大学中药学院,辽宁沈阳110016

出　　处：《中国中药杂志》2015年第4期700-703,共4页China Journal of Chinese Materia Medica

基　　金：辽宁省教育厅创新团队项目(LT2012018);辽宁省科技厅项目(2011412004-5)

摘　　要：采用高效液相色谱（HPLC）的方法,以Diamonsil C18色谱柱（4.6 mm250 mm, 5 μm）,流动相为乙腈-体积分数为0.15%的磷酸水,梯度洗脱,流速为1.0 mLmin-1,柱温为30 ℃,检测波长365 nm的色谱条件同时测定了哥王中杨梅素、木犀草素、芹菜素和山柰酚含量.各成分之间分离度良好,杨梅素、木犀草素、芹菜素、山柰酚的质量浓度分别在0.100 8~1.008（r=0.999 2）,0.484 8~4.848（r=0.999 0）,1.354~13.54（r=0.999 6）,0.316 8~3.168 mgL-1（r=0.999 0）内与峰面积呈良好的线性关系,平均回收率分别为98.5%（RSD=1.2%）, 100.9%（RSD=1.7%）, 99.7%（RSD=0.81%）, 98.9%（RSD=1.6%）.表明所建立的方法快速简便、具有较好的重复性和稳定性,为了哥王药材的质量控制提供参考.The HPLC method was established to simultaneously determine the contents of myricetin, luteolin, apigenin and kaempferol in Wikstroemia indica （L.） C. A. Mey.. The method was carried out on a Diamonsil C18 column （4.6 mm250 mm, 5 μm） eluted with the mobile phases of water containing 0.15% phosphoric acid and acetonitrile in gradient mode. The UV detection wavelength was 365 nm. The flow rate was 1.0 mLmin-1 and the column temperature was set at 30 ℃. All the standard compounds showed a good linearity in the range of 0.100 8-1.008 （r=0.999 2）, 0.484 8-4.848 （r=0.999 0）, 1.354-13.54 （r=0.999 6）, 0.316 8-3.168 mgL-1（r=0.999 0） for myricetin, luteolin, apigenin and kaempferol, respectively. The average recoveries of these four flavonoids were 98.5%, 100.9%, 99.7% and 98.9% with RSD 1.2%, 1.7%, 0.81% and 1.6%, respectively. In conclusion, the method is simple, rapid and accurate. It can be applied for the quality control of Wikstroemia indica.keywords：Wikstroemia indica

关 键 词：了哥王 高效液相色谱法 含量测定 

分 类 号：R284.2[医药卫生—中药学] O657.72[医药卫生—中医学]