白细胞五分类的光学信号检测与处理系统设计  

Optical Signal Detection and Processing System for WBC Five Classification

作　　者：万里霞[1] 龙伟[1] 张星原[1] 卢斌[1] 

机构地区：[1]南昌大学信息工程学院,江西南昌330031

出　　处：《激光与光电子学进展》2015年第3期216-222,共7页Laser & Optoelectronics Progress

基　　金：国家自然科学基金(61261011)

摘　　要：在五分类血液分析仪研发领域中,虽然白细胞的分类方式多样,仪器的功能也相对比较完善,但大多数仪器的结构设计复杂,外部干扰因素多,稳定性和精密度难以得到保障。为此,提出了一种应用于白细胞五分类的信号检测与处理系统,该系统采用全光学技术对细胞进行参数检测,将得到的前侧光散射量(FSC)与轴向光吸收量(ALL)作为细胞的特征量,并设计出光电转换电路、滤波及放大电路和模拟/数字(A/D)转换电路,最终完成了对白细胞的五分类工作,采用该技术的血液分析仪样机具有结构简单,可靠性高的特点。测试结果表明,样机对白细胞分类的相关性r较好,且仪器的精密度与国外高档五分类血液分析仪Mythic 22接近,仪器的功能和性能比较完善。There are many white blood cell （WBC） classification methods in the research and development field of five classification blood analyzer and their functions are relatively perfect, but the majority of instrument structure is complex, and has more interference factors, and thus their stability and precision can not be guaranteed as a result. Therefore, an optical signal detection and processing system that is applied to the WBC five classification and uses the full-optical detection technology is proposed, taking the front side light scattering （FSC） and axial light absorption （ALL） as characteristics of cells, the photoelectric conversion circuit, filter and amplification circuit and analog/digital （A/D）conversion circuit are designed, and the five classification of WBC is completed. The blood analyzer using this system has the advantages of simple structure and high reliability. The test results show that the correlation r of the system for WBC classification is good, and the precision of the instrument is closed to Mythic 22, which is a high-grade five classification blood cell analyzer, and the function and performance of the instrument is perfect.

关 键 词：医用光学 血液分析仪 五分类 全光学检测 特征量 相关性 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]