2009～2013年广西H1N1和H3N2亚型猪流感病毒血清学调查  

Serological investigation of H1N1 and H3N2 subtype swine influenza virus in Guangxi from 2009 to 2013

作　　者：陈樱[1] 於庆雄 银凤桂 薛辉[1] 李法凯 莫彦宁[1] 肖雄[1] 杨文娟[1] 韦祖樟[1] 刘芳[1] 蹇慧[1] 黄伟坚[1] 

机构地区：[1]广西大学动物科学技术学院,南宁530005

出　　处：《南方农业学报》2015年第1期155-159,共5页Journal of Southern Agriculture

基　　金：国家现代化农业产业技术体系广西创新团队生猪产业功能专家经费项目(nycytxgxcxtd-03-15-1);广西高校科学技术研究项目(YB2014005);广西大学科研基金项目(XJZ140236)

摘　　要：摘要：【目的】了解广西H1N1和H3N2亚型猪流感病毒（SIV）的存在形势及其流行规律，为下一步有效监测和防控猪流感（SI）提供理论依据和原始溯源。【方法】对2009～2013年于广西11个地级市的屠宰场、规模化养猪场和个体养殖户采集的1170份猪血清进行血凝抑制（Hr）试验检测，统计欧洲禽源H1N1（EA．H1N1）亚型和新型人源重组H3N2（Hu．H3N2）亚型4株毒株（LB144和BB2、NNXD和JGB4）的抗体阳性率，并分析H1N1和H3N2亚型毒株的存在形势及其流行规律。【结果】2009-2013年，EA．H1N1亚型LB144毒株的平均抗体阳性率高达20．68％，且保持稳定，是广西地区主要的流行毒株；Hu．H3N2亚型JGB4毒株从2010年开始流行，且呈逐渐蔓延的趋势，平均抗体阳性率为19．96％。广西北海、南宁、玉林、贺州、桂林、贵港和柳州市受到EA—H1N1和Hu—H3N2亚型毒株多重感染，且感染程度较严重；百色、河池和防城港市的感染程度相对较低。育肥猪最易感染SIV，尤其是BB2和JGB4毒株，其抗体阳性率分别达66．00％和40．00％。EA—H1N1亚型毒株间的交叉感染阳性率（21．52％）略低于Hu—H3N2亚型毒株间的交叉感染阳性率（27．45％），而两株不同亚型毒株交叉感染时以BB2与JGB4毒株的交叉感染阳性率最高（16．23％），3株交叉感染时以BB2、NNXD和JGB4毒株间的交叉感染阳性率最高（11．60％），4株同时交叉感染阳性率也有6．96％。【结论】广西猪群已普遍存在EA-H1N1和Hu．H3N2亚型毒株混合感染的现象，尤其是地处两省边界上的地级市感染更严重，并以育肥猪和保育猪较易感。因此在sI防控工作中，除做好哺乳仔猪和种猪的日常管理外，还应减少保育猪的外来应激，适当调整育肥猪的饲养密度，以减少新型SIV重组毒株的产生。[Objective]The existing situation and epidemic law of H1N1 and H3N2 subtype swine influenza viruses （SIV） in Guangxi was investigated in order to provide theoretical basis and original trace for monitoring and preventing SIV. [＇Method]The 11700 sera samples were collected from slaughterhouses, large-scale piggeries and individual farmers of 11 prefecture-level cities in Guangxi during 2009-2013 and then detected by hemagglutination inhibition （HI） tests. The antibody positive rates of European avian H1N1 subtype （EA-H1N1） and novel human recombinant H3N2 subtype （Hu-H3N2） subtype strains viz., LB144, BB2, NNXD and JGB4 were analyzed to investigate their existing situations and epidemic laws. [Result]The EA-H1N1 subtype strain LB144 remained stable and became the predominant strain during 2009-2013, and its average antibody positive rate reached to 20.68%. Since the Hu-H3N2 subtype strain JGB4 began to prevail in 2010 and showed gradually spread tendency, and its average antibody positive rate was 19.96%. Baihai, Nanning, Yulin, Hezhou, Guilin, Guigang and Liuzhou in Guangxi suffered from multi-infection of Hu-H3N2 and EA-H1N1 subtype strains and their degrees of infection were more serious, while that of Baise, Hechi and Fangchenggang were relatively lower. Moreover, the fattening pig were the most susceptible to SIV, especially infecting BB2 and JGB4, and their antibody positive rates reached to 66.00% and 40.00%, respectively. The cross-infection positive rate between EA-H1N1 subtype strains （21.52%） was lower than that between Hu H3N2 subtype strains （27.45%）. The cross-infection positive rate between BB2 and JGB4 strains was the highest （16.23%） among any two defferent subtype strains. The cross-infection positive rate among BB2, NNXD and JGB4 was the highest （11.60%） among any three defferent strains. There was also cross-infection positive rate of 6.96% among four strains. [Conclusion]There exists widespread phenomenon of mixed infection among EA-H1N1 and Hu- H3N2 subtype s

关 键 词：猪流感病毒(SIV) H1N1亚型 H3N2亚型 血清学调查 重组毒株 

分 类 号：S858.28[农业科学—临床兽医学]