γ-干扰素释放试验在肺结核诊断中的应用  

Application of interferon gamma release test in the diagnosis of pulmonary tuberculosis

作　　者：王长征[1] 张平安[1] 梅冰[2] 曲仕林 李滔[2] 

机构地区：[1]武汉大学人民医院检验科,武汉430060 [2]华中科技大学同济医学院附属荆州医院检验医学部 [3]荆州市胸科医院

出　　处：《公共卫生与预防医学》2015年第1期49-52,共4页Journal of Public Health and Preventive Medicine

基　　金：国家临床重点专科建设项目(财社[2010]3015)

摘　　要：目的探讨γ-干扰素释放试验在肺结核诊断中的应用价值。方法用γ-干扰素释放试验试剂盒(WT-IGRA)定量检测65例肺结核病患者、45例非肺结核患者和20例体检健康者外周血结核特异性γ-干扰素的含量,同时与结核菌素纯化蛋白质衍生物(PPD)试验及结核蛋白芯片(TB-Ig G)检测进行平行比较分析。结果 WT-IGRA的敏感度、特异度、阳性预测值、阴性预测值均为89.23%,Youden指数78.46%,ROC曲线下面积0.89,诊断价值优于PPD试验及TB-Ig G检测。结论 WTIGRA的应用将有助于提高肺结核感染的阳性检出率,可作为临床肺结核诊断重要的辅助手段之一。Objective To investigate the clinical application of IFN-γ release test in diagnosis of pulmonary tuberculosis. Methods The concentration of mycobacterium tuberculosis specific IFN-γ in peripheral blood of 65 patients with pulmonary tuberculosis,45 patients with non- tuberculosis and 20 cases of normal physical examination were detected by the IFN-γ release test reagents（ WTIGRA）,and the results were parallel comparatively analyzed with purified protein derivative of tuberculin（ PPD） test and tuberculosis protein chip（ TB-Ig G）. Results The specificity,sensitivity,positive predictive value,negative predictive value and of WT-IGRA were all 89. 23%,Youden index was 78. 46%,and the following ROC curve was 0. 89. The diagnostic value of WT-IGRA was better than the PPD test and TB-Ig G test. Conclusion WT-IGRA can improve the positive rate of tuberculosis infection diagnosis,and can be used as an important clinical auxiliary diagnosis for pulmonary tuberculosis.

关 键 词：Γ-干扰素释放试验 肺结核 诊断 

分 类 号：R521[医药卫生—内科学]