实时荧光PCR法快速检测耐甲氧西林金黄色葡萄球菌  

Rapid detection of methicillin-resistant staphylococcus aureus by Real-time PCR

作　　者：聂署萍[1] 钟伟传 黄烈[1] 吴润香[1] 朱新建[1] 凌利芬[1] 

机构地区：[1]广东医学院附属福田医院,广东深圳518033 [2]深圳市龙华新区人民医院

出　　处：《临床血液学杂志（输血与检验）》2015年第1期117-119,共3页Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)

摘　　要：目的:评价一种直接从标本中快速检测耐甲氧西林金黄色葡萄球菌(MRSA)的实时荧光PCR方法。方法:临床送检标本中筛选出46份金黄色葡萄球菌阳性的标本,进行实时荧光聚合酶链反应(PCR)检测,并与全自动细菌鉴定仪(VITEK-2Compact)/头孢西丁纸片扩散法进行比较,评价实时荧光PCR方法的敏感性及特异性。结果:VITEK-2Compact分离出金黄色葡萄球菌46株,其中MRSA 12株,与头孢西丁纸片扩散法结果一致。实时荧光PCR法检测出金黄色葡萄球菌46株,其中MRSA 13株,敏感性及特异性分别为100%(95%CI,75.75,100),97.06%(95%CI,85.08,99.48)。2种方法有很好的一致性[Kappa=0.945(95%CI,0.839,1.000)]。结论:实时荧光PCR法是一种能从标本中直接检测MRSA的快速,敏感的方法。Objective：To evaluate a rapid method for the detection and identification of methicillin-resistant Staphylococcus aureus（MRSA）from clinical specimens.Method：46Staphylococcus aureus positive clinical samples were detected by real-time PCR.VITEK 2Compact and cefoxitin disk diffusion method were used as golden standard to evaluate the sensitivity and specificity of the real-time PCR method.Result：VITEK 2Compact and cefoxitin disk diffusion method identified 46 Staphylococcus aureus strains,of which 12 strains were MRSA.Realtime PCR detected 46 Staphylococcus aureus,including 13 MRSA.The sensitivity and specificity were 100%（95% CI,75.75,100）and 97.06%（95% CI,85.08,99.48）,respectively.Real-time PCR method showed very good consistency with the reference method[Kappa=0.945（95% CI,0.839,1.000）].Conclusion：Real-time PCR method may be a reliable and fast way to detect and identify MRSA directly from the clinical specimen.

关 键 词：耐甲氧西林金黄色葡萄球菌 实时荧光PCR 医院感染 

分 类 号：R378.1[医药卫生—病原生物学]