芋花粉活力测定及最佳贮藏方法的选择  

Determination of Taro Pollen Viability and Choice of the Best Storage Method

作　　者：孙亚林[1] 柯卫东[1] 黄新芳[1] 董红霞[1] 张龙辉[2] 何燕红[2] 

机构地区：[1]武汉市蔬菜科学研究所,武汉430065 [2]园艺植物生物学教育部重点实验室/华中农业大学园艺林学学院,武汉430070

出　　处：《中国农学通报》2015年第4期62-67,共6页Chinese Agricultural Science Bulletin

基　　金：科技部"十二五"国家科技支撑技术计划项目"水生蔬菜高效生产技术研究与示范"(2012BAD-2700);武汉市青年科技晨光计划"芋杂交技术与遗传规律的研究"(201271031406)

摘　　要：为确保芋杂交育种实验的有效开展,研究芋花粉活力测定和花粉贮藏的方法。实验结果表明,柱头稀释液培养法和培养基萌发法均能有效测定芋的花粉活力;芋花粉萌发的最佳培养基配方是:10 mg/L硼酸+0.01%无水氯化钙+5%蔗糖。芋花粉的寿命短,但不同芋品种的花粉寿命不同;芋花粉在15%空气湿度或-20℃干燥条件下可以贮藏相对较长的时间。The methods to test pollen viability and to store the pollen of taro were studied in order to ensuretaro crossbreeding experiment. The results showed that both stigma dilution culture method and mediumculture method could be used to test the pollen viability of taro, and the best medium of pollen germination was10 mg/L boric acid ＋0.01% calcium chloride anhydrous＋ 5% sucrose. Taro pollen had short life, but differentvarieties of taro had different pollen viability. Pollen could be stored longer in the 15% air humidity and-20℃under drying.

关 键 词：芋 离体培养 花粉活力 花粉贮藏 

分 类 号：S632.3[农业科学—蔬菜学]