聚乳酸-聚三亚甲基碳酸酯/GDNF导管对大鼠脊髓损伤区GAP-43mRNA表达的影响  

The influence of PLA-Poly-PTMC/GDNF on the protein expression of GAP-43 mRNA in spinal cord injury of rats

作　　者：邵国喜[1] 焦晶雪[1] 刘钦毅[1] 程兆华[1] 李伟民[1] 

机构地区：[1]吉林大学第二医院骨科,吉林长春130041

出　　处：《中国实验诊断学》2015年第2期198-201,共4页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省科技厅国际合作课题资助(20120721)

摘　　要：目的探讨PLA-PTMC/GDNF复合导管对脊髓损伤功能恢复的影响。方法我们采用RT-PCR的方法对GAP-43的mRNA表达进行分析,从基因水平上来检测植入PLA-PTMC/GDNF复合导管与其他治疗组GAP-43基因表达上的差异。结果术后第2周时,A组、B组、C组和D组的GAP-43mRNA相对表达水平与正常组相比明显增高;术后4周时,B组和D组的GAP43mRNA表达与第2周时相比明显增高,与A组和C组相比明显增高,有显著性差异;术后6周时,D组的GAP-43mRNA表达仍明显增高,与A组、B组和C组相比增高有显著性差异;第8周时各组GAP-43mRNA表达均有所下降,但D组的GAP-43mRNA相对表达水平与A组、B组和C组相比仍高,有显著性差异。结论 PLA-PTMC/GDNF复合导管更有利于轴突再生的修复连接和脊髓损伤的功能恢复。Objective To explore the influence of PLA-Poly-PTMC/GDNF on the mRNA expression of GAP-43 in spinal cord injury of rats.Methods To explore the mRNA expression of GAP-43 at molecular biological level with RTPCR to make certain the difference between PLA-PTMC/GDNF and others.Results A,B,C,D groups showed high protein expression at 2 weeks,and there is no significant difference among these groups.B,D groups had significant higher expression at 4weeks than that of two weeks.D group showed high protein expression at 6weeks than other groups and all groups showed higher expression.At 8weeks,all groups showed lower expression of GAP-43 mRNA,but D group showed more expression than others.Conclusion PLA-Poly-PTMC/GDNF is more helpful for axon regeneration spinal cord recovery.

关 键 词：脊髓损伤 聚乳酸-聚三亚甲基碳酸酯 GAP-43mRNA 导管移植 

分 类 号：R651.2[医药卫生—外科学]