大豆种子发芽期耐旱性鉴定的适宜PEG-6000浓度筛选  

Screening of Appropriate PEG-6000 Concentration for the Identification of Soybean Drought Tolerance at Germination Stage

作　　者：舒英杰[1] 周玉丽[1] 时侠清[1] 胡能兵[1] 邵庆勤[1] 杜军利[1] 

机构地区：[1]安徽科技学院农学院,安徽凤阳233100

出　　处：《大豆科学》2015年第1期56-59,共4页Soybean Science

基　　金：安徽省教育厅高等学校省级优秀青年人才基金(2012SQRL143);国家自然科学基金(31101212)

摘　　要：以抗旱性不同的3个大豆品种（湘豆3号、Lee68和宁镇1号）为试材,研究了不同浓度（5%、10%、15%、20%、25%和30%）的PEG-6000模拟发芽期干旱胁迫对大豆种子发芽、子叶苗膜脂过氧化以及渗透调节物质含量的影响。结果表明：10%以下的PEG-6000能起引发作用,促进大豆种子的萌发,显著提高发芽势和发芽率;随着PEG-6000浓度的升高,大豆种子的发芽明显受抑制,当PEG-6000浓度大于25%时抑制更明显,发芽势、发芽率、发芽指数和简易活力指数显著下降;随着PEG-6000浓度的升高,大豆子叶苗中的丙二醛（MDA）、脯氨酸（Pro）、可溶蛋白和可溶性糖含量显著增加,PEG-6000浓度在15%-25%间增加幅度最大,PEG-6000浓度小于15%或大于25%时增加不明显。综合种子发芽、子叶苗的膜脂过氧化程度以及渗透调节物质含量的变化,可初步确定室内采用PEG-6000浸种法模拟干旱胁迫鉴定大豆发芽期耐旱性的适宜浓度为20%-25%。Three soybean varieties（ Ningzhen 1,Lee68 and Xiangdou 3） were used to study the effect of different PEG-6000concentrations（ 5%,10%,15%,20%,25% and 30%） on germination of soybean seed,membrane lipid peroxidation and osmotic substances of soybean seedlings. Results showed that when the PEG-6000 concentration less than 10%,which can promote soybean seeds germination,improve the germination potential and germination rate significantly. When the PEG-6000 concentrations were more than 25%,with the increasement of PEG-6000 concentration,germination potential,germination rate,germination index and simple vigor index of soybean seed decreased significantly. The contents of malondialdehyde（ MDA）,proline（ Pro）,soluble protein and soluble sugar increased significantly with the PEG-6000 concentration increased,especially when the PEG-6000 concentration was between 15% and 25%. We can conclude preliminarily that the appropriate PEG-6000 concentration for the identification of soybean drought tolerance in seed germination stage was 20%-25% by synthesizing seed germination,membrane lipid peroxidation and the content change of osmotic substances.

关 键 词：大豆 PEG-6000 干旱胁迫 发芽期 耐旱性 

分 类 号：S565.1[农业科学—作物学]