AMP对铝胁迫诱导丹波黑大豆柠檬酸分泌及其铝耐受性的影响  

Effects of AMP on the Al-induced Citrate Exudation and Al-resistance of Tamba Black Soybean

作　　者：郭传龙[1] 赵艳[1] 武孔焕[1] 李昆志[1] 陈丽梅[1] 

机构地区：[1]昆明理工大学生命科学与技术学院生物工程技术研究中心,云南昆明650500

出　　处：《大豆科学》2015年第1期82-86,共5页Soybean Science

基　　金：国家自然科学基金(31260063)

摘　　要：为了考察AMP(Adenosine 5’-monophosphate)对铝耐受型丹波黑大豆(RB)柠檬酸的分泌及铝抗性的影响,在50 mmol·L-1铝溶液中添加100 mmol·L-1AMP共处理RB,结果表明RB根的相对生长率显著小于单独用50 mmol·L-1铝胁迫处理的植株。免疫共沉淀分析显示铝胁迫下AMP的存在降低RB根中14-3-3蛋白与磷酸化质膜H+-ATP酶的相互作用,使根尖质膜H+-ATP酶活性降低约1倍,氢泵活性和H+分泌能力显著降低,根柠檬酸的分泌量减少约1倍。说明铝胁迫下AMP通过抑制14-3-3蛋白和质膜H+-ATP酶的互作来减少RB根尖的氢泵活性和柠檬酸分泌作用,从而降低RB根的相对生长率及其对铝胁迫的耐受性。To investigate the effect of Adenosine 5＇-monophosphate（ AMP） on the citrate secretion and Al-resistance of Alresistant Tamba black soybean（ RB）. RB was cotreated with AMP and 50 mmol·L^-1Al. The results showed that the relate root growth rate of RB co-treated with 100 mmol·L^-1AMP and 50 mmol·L^-1Al for 8 h was significantly lower than that of the plants treated with 50 mmol · L^-1Al alone. Co-immunoprecipitation showed that the presence of AMP under Al stress decreased the Al-enhanced interaction of PM H＋-ATPase and 14-3-3 protein in root tips of RB,which reduced PM H＋-ATPase activity by approximately 1-fold and thereby led to a significant decrease in H＋efflux and citrate secretion（ approximately 1-fold） in RB roots. Those results indicated that AMP decreases H＋activity and citrate exudation in RB via its inhibition on the interaction between 14-3-3 protein and PM H＋-ATPase under Al stress,thereby reducing the relate root growth rate of RB and its Al-tolerance.

关 键 词：铝胁迫 丹波黑大豆 铝诱导的柠檬酸分泌 14-3-3蛋白 质膜H+-ATP酶 

分 类 号：Q945.78[生物学—植物学]