大豆种子寄藏真菌致病性测定方法比较  

Comparison on Methods for Pathogenicity Test of Seed-Borne Fungi in Soybean

作　　者：李雪光[1,2] 刘志勇 战丽莉[1,3] 宋洁[1,2] 鲁建聪 许艳丽[1] 

机构地区：[1]中国科学院东北地理与农业生态研究所/黑土区农业生态院重点实验室,黑龙江哈尔滨150081 [2]东北农业大学农学院,黑龙江哈尔滨150030 [3]黑河学院物理化学系,黑龙江黑河164300

出　　处：《大豆科学》2015年第1期93-98,共6页Soybean Science

基　　金：国家"十一五"科技支撑计划(2006BAD21B01-15)

摘　　要：寄藏真菌可侵染大豆种子,降低种子的萌芽及活力,影响种子的品质和商品等级。为探讨不同接种方法对寄藏真菌致病性的影响,在实验室条件下比较了种子接种法和植株接种法(切顶端接种法、伤口接种法、切茎接种法和下胚轴接种法)对寄藏真菌致病性的影响。结果表明:各方法中寄藏真菌均对大豆产生致病性,并能显著区分寄藏真菌间致病性差异。其中切顶端接种法相比其他3种植物接种方法更简单有效而准确,而种子接种法更适合在室内进行,且周期短,相比植株接种法方便快捷。Seed-borne fungi could decrease germination,activity,quality and commercial grade by infecting soybean seed.For discussing the effect of different inoculation methods on pathogenicity of seed-borne fungi,seed inoculation method and plant inoculation methods（ apical cut inoculation method,wound inoculation method,stem cut inoculation method and hypocotyl inoculation method） were compared at laboratory condition. The result showed that seed-borne fungi could infect soybean under each inoculation method. The differences of pathogenicity between seed-borne fungi were significantly distinguished by all inoculation methods. Compared with other three plant inoculation methods,apical cut inoculation method was simple,effective and accurate. Seed inoculation method was fit for room test and the test cycle was short. It was also more convenient and efficient than plant inoculation.

关 键 词：种子病害 寄藏真菌 大豆 致病性测定 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]