一种改良的大豆DNA提取方法  

A Modified UREA Protocol for Soybean DNA Extraction

作　　者：吴艳艳[1] 代德艳 蔡春梅[1] 

机构地区：[1]青岛农业大学生命科学学院/山东省高校植物生物技术重点实验室,山东青岛266109

出　　处：《大豆科学》2015年第1期112-115,共4页Soybean Science

基　　金：国家自然科学基金(31071444;31371647);山东省优秀中青年科学家科研奖励基金(BS2011NY006);青岛农业大学高层次人才启动基金(630908;631304)

摘　　要：大豆组织中含有较多的蛋白质、糖类、酚类和脂类物质,要从中提取高质量的DNA比较困难。针对这一情况提出一种改良UREA大豆DNA提取方法(m UREA),该方法用异丙醇沉淀DNA,并配制washing buffer洗涤DNA沉淀。并以大豆叶片和种子为材料,将m UREA法与CTAB法和SDS法进行对比。结果表明,以大豆叶片为材料时,m UREA法提取的DNA产率最高,质量最好;以大豆种子为材料时,m UREA法提取的DNA产率略低于SDS法,但纯度最高。综合来看,m UREA法是一种高效的大豆基因组DNA提取法,从大豆种子和叶片中提取的DNA浓度和纯度都较高,能满足后续各种分子生物学的要求。It is difficult to extract high quality and sufficient yield of genomic DNA for soybean molecular biological research,because of a great deal of protein,polysaccharides,hydroxybenzenes and esters. A modified UREA（ m UREA） protocol for extracting soybean DNA was provided in this paper. We precipitated DNA with isopropanol and washed DNA pellet with washing buffer. Using soybean leaves and seeds as material,compared m UREA method with CTAB and SDS methods. The results showed that the quality and yield of DNA from soybean leaves were highly significant than using m UREA method. And using soybean seeds as material,the purity of DNA from m UREA was better than the other two methods. So m UREA method yielded much more DNA of high quality for soybean leaves and seeds that was suitable for various molecular operations.

关 键 词：改良UREA法 大豆 基因组DNA提取 

分 类 号：S565.1[农业科学—作物学]