替莫唑胺对神经胶质瘤TJ905细胞增殖特性和迁移能力的影响  

Effect of temozolomide on cell proliferation and migration ability of glioma TJ905 cells

作　　者：于喜贞[1,2,3] 韩光魁[2] 杨文涛[1] 李正友[2] 靳峰[2] 

机构地区：[1]新乡医学院,河南新乡453003 [2]济宁医学院附属医院神经外二科,山东济宁272029 [3]济宁医学院病理生理学实验室,山东济宁272029

出　　处：《新乡医学院学报》2015年第2期111-114,共4页Journal of Xinxiang Medical University

基　　金：国家自然科学基金资助项目(编号:8107779);山东省中青年科学家科研奖励基金资助项目(编号:BS2010YY006)

摘　　要：目的探讨替莫唑胺(TMZ)对人胶质瘤TJ905细胞增殖和迁移能力的影响。方法培养TJ905细胞,分别应用不同浓度的TMZ(0、50、100、200、400μmol·L-1)干预TJ905细胞,干预不同时间(24、48、72 h)后收集细胞,应用Cell Counting Kit-8检测药物干预前后细胞增殖活性变化;细胞划痕实验检测400μmol·L-1TMZ对TJ905细胞迁移能力的影响。结果随着TMZ浓度的升高和培养时间的延长,TJ905细胞的密度降低,细胞出现皱缩,增殖受到明显抑制,并可见散在的细胞碎片;TJ905细胞存活率随着培养液中TMZ浓度的升高和培养时间的延长而降低(P<0.05);400μmol·L-1TMZ的培养液培养24、48和72 h后TJ905细胞迁移度均低于对照组(P<0.05)。结论 TMZ能有效抑制TJ905细胞增殖和迁移。Objective To explore the effect of temozolomide（ TMZ） on the proliferation and migration of glioblastoma TJ905 cells. Methods Glioblastoma TJ905 cells were cultured. The cells were intervened by different TMZ concentrations（ 0,50,100,200,400 μmol·L- 1） at different time（ 24,48,72 h）,then the cells were collected to detect the change of cell cycle and proliferation with Cell Counting Kit-8; the effect of 400 μmol·L- 1TMZ on cell migration was assessed by cell scuffing test. Results With TMZ concentration increasing and the extension of incubation time,the density of the TJ905 cells was reduced,the morphology of TJ905 cells was shrinkaged,proliferation was significantly inhibited,and the scattered debris could be seen; survival rate of glioma TJ905 cells was decreased with elevated TMZ concentration and lengthened cultivation time（ P〈0. 05）; level of TJ905 cell migration was lower than that in control group after being cultured with 400 μmol·L- 1TMZ solution for 24,48 and 72 h（ P〈0. 05）. Conclusion The proliferation of glioma TJ905 cells can be inhibited by inhibiting the proliferation speed and migration ability with TMZ.

关 键 词：替莫唑胺 TJ905 胶质瘤细胞 细胞增殖 迁移 

分 类 号：R979.1[医药卫生—药品]