Preparation of Surface-enhanced Raman Scattering（SERS）-active Optical Fiber Sensor by Laser-induced Ag Deposition and Its Application in Bioidentification of Biotin/Avidin  

作　　者：LIU Chunyu WANG Shaoyan FU Cuicui LI Haibo XU Shuping XU Weiqing 

机构地区：[1]State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University, Changchun 130012, R R. China [2]College of Science, Changchun University of Science and Technology, Changchun 130022, P. R. China

出　　处：《Chemical Research in Chinese Universities》2015年第1期25-30,共6页高等学校化学研究（英文版）

基　　金：Supported by the National Natural Science Foundation of China（Nos.91027010, 21073073, 20903043, 20973075）, the Research Fund for the Doctoral Program of Higher Education of China（No.20090061120089） and the National Instrumentation Program（NIP） of the Ministry of Science and Technology of China（No.2011YQ03012408）.

摘　　要：A surface-enhanced Raman scattering（SERS） optical fiber sensor was prepared by the laser-induced deposition ofAg nanoparticle membrane on a silica optical fiber tip, which was applied to the real time SERS spectral monitoring on the biorecognition of biotin/avidin. The bioidentification of biotin/avidin was carried out through a indirect method, in which the bioidentification is based on the SERS response signal of a labeled dye（Atto610） after its fluorescence has been quenched totally by the deposited Ag nanoparticle membrane. By SERS monitoring the bioidentification process of biotin/avidin, it has been found that this recognition process is finished in 40 min. The lowest detection concentration of biotin is 1.0 × 10^-7 mg/mL. This research is promising in the application of immunoassays on line and in vivo.A surface-enhanced Raman scattering（SERS） optical fiber sensor was prepared by the laser-induced deposition ofAg nanoparticle membrane on a silica optical fiber tip, which was applied to the real time SERS spectral monitoring on the biorecognition of biotin/avidin. The bioidentification of biotin/avidin was carried out through a indirect method, in which the bioidentification is based on the SERS response signal of a labeled dye（Atto610） after its fluorescence has been quenched totally by the deposited Ag nanoparticle membrane. By SERS monitoring the bioidentification process of biotin/avidin, it has been found that this recognition process is finished in 40 min. The lowest detection concentration of biotin is 1.0 × 10^-7 mg/mL. This research is promising in the application of immunoassays on line and in vivo.

关 键 词：Process monitoring Photochemical method Laser-inducement SERS-active optical fiber Biotin/avidin Specific identification 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置] TS253.7[自动化与计算机技术—控制科学与工程]