Studies on the Interaction of Novel Organogermanium Sesquioxides with DNA  

作　　者：GE Shun ZHAO Ying SUI Beibei SHANGGUAN Guoqiang 

机构地区：[1]School of Biological Science, Jining Medical University, Rizhao 276826, P. R. China

出　　处：《Chemical Research in Chinese Universities》2015年第1期31-37,共7页高等学校化学研究（英文版）

基　　金：Supported by the Natural Science Foundation of Shandong Province, China（No.ZR2011HL003）.

摘　　要：The affinity and mode of interaction of four novel organogermanium sesquioxides with calf thymus DNA（CT-DNA） and two synthetic oligonucleotides, d（AT）22d（AT）22 and d（GC）22d（GC）22, were investigated by a combination of absorption spectroscopy, DNA thermal denaturalization method, viscosity method, fluorometric technique, and competitive binding study with ethidium bromide（EB）. The results show that the organogermanium compounds can interact with DNA by intercalation, the binding ability of the compounds to CT-DNA and the synthetic oligonucleotides was found to be modest（in comparison to the proven intercalators）, With binding constants on the order of 10^3-10^5 L/mol, respectively. Generally, the binding of the organogermanium sesquioxides with naphthalene moiety to DNA is stronger than that of the compounds with anthraquinone moiety. And the compounds with anthraquinone moiety have preference for binding to AT-rich duplexes, whereas the compounds with naphthalene moiety have a little preference for binding to GC-rich duplexes. DNA may be the primary effect target.The affinity and mode of interaction of four novel organogermanium sesquioxides with calf thymus DNA（CT-DNA） and two synthetic oligonucleotides, d（AT）22d（AT）22 and d（GC）22d（GC）22, were investigated by a combination of absorption spectroscopy, DNA thermal denaturalization method, viscosity method, fluorometric technique, and competitive binding study with ethidium bromide（EB）. The results show that the organogermanium compounds can interact with DNA by intercalation, the binding ability of the compounds to CT-DNA and the synthetic oligonucleotides was found to be modest（in comparison to the proven intercalators）, With binding constants on the order of 10^3-10^5 L/mol, respectively. Generally, the binding of the organogermanium sesquioxides with naphthalene moiety to DNA is stronger than that of the compounds with anthraquinone moiety. And the compounds with anthraquinone moiety have preference for binding to AT-rich duplexes, whereas the compounds with naphthalene moiety have a little preference for binding to GC-rich duplexes. DNA may be the primary effect target.

关 键 词：Organogermanium sesquioxide DNA INTERACTION INTERCALATION 

分 类 号：O627.42[理学—有机化学] Q523[理学—化学]