毒蕈碱受体及其亚型对四磨汤诱发胃窦平滑肌收缩的差异性影响  

Different effects of muscarinic receptor and subtypes on Simo Decoction-induced contraction of gastric antrum smooth muscle

作　　者：钱伟[1] 戴迟兵[2] 刘娜[1] 侯晓华[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院,湖北武汉430022 [2]三峡大学附属第二临床医学院/仁和医院,湖北宜昌443000

出　　处：《药物评价研究》2015年第1期36-39,共4页Drug Evaluation Research

基　　金：国家973计划项目(2009CB523002)

摘　　要：目的探讨毒蕈碱(M)受体及其亚型对四磨汤(SMD)诱发胃窦纵、环行肌收缩的差异性影响。方法 40只雄性SD大鼠,随机分为5组,每组8只,分别为:单纯SMD组、阿托品(Atropine)+SMD组、加拉明(Gallamine)+SMD组、4-二苯乙酰氧基-N-甲基哌淀甲碘化物(4-DAMP)+SMD组及加拉明+4-DAMP+SMD组。制备胃窦纵、环行平滑肌条,分别采用累积量加入四磨汤,以及阿托品(10-6 mol/L)、加拉明(10-6 mol/L)、4-DAMP(0.4×10-6 mol/L)、加拉明+4-DAMP孵育肌条后再累积加入四磨汤,记录平滑肌等长收缩活动,比较纵、环行肌收缩的差异性。结果四磨汤诱发胃窦纵、环行肌收缩活动增加,但两者间无明显差异(P>0.05),非选择性M受体阻断剂阿托品及选择性M2受体阻断剂加拉明对四磨汤诱发胃窦环行肌收缩活动的抑制明显强于纵行肌(P<0.05),选择性M3受体阻断剂4-DAMP及加拉明+4-DAMP对四磨汤诱发胃窦纵行肌收缩活动的抑制作用明显强于环行肌(P<0.05)。结论 M受体及其亚型参与了四磨汤对胃窦运动的调节,胃窦环行肌收缩更多的依赖于M受体及其M2亚型的激活,胃窦纵行肌收缩更多的依赖于M3受体亚型的激活。Objective To investigate the different effects of muscarinic（M） receptor and its subtype on Simo Decoction（SMD）-induced contraction of antral longitudinal and circular strips. Methods Forty SD rats were randomly divided into five groups including SMD, Atropine ＋ SMD, Gallamine ＋ SMD, 4-DAMP ＋ SMD, and Gallamine ＋ 4-DAMP ＋ SMD groups, the spontaneous contraction of antral longitudinal and circular strips induced by SMD were noted in the organ bath in the presence or absence of atropine, Gallamine, 4-DAMP or Gallamine ＋ 4-DAMP. Results SMD induced the contraction of longitudinal and circular strips increasing with no significant difference（P〉0.05）, SMD-induced contractile activity of longitudinal strips after incubation with non-selective M receptor blocker atropine or selective M2 receptor blocker gallamine was stronger than circular strips（P〈0.05）, and after incubation with selective M3 receptor blocker 4-DAMP or gallamine ＋ 4-DAMP, SMD-induced contractility of cicular strips was stronger than longitudinal strips（P〈0.05）. Conclusion SMD-induced contraction of antral circular strips is more dependent on activation of M and M2 receptor than longitudinal strips. However, SMD-induced contraction of antral longitudinal strips is more dependent on activation of M3 receptor than circular strips.

关 键 词：毒蕈碱受体 四磨汤 胃窦 平滑肌 收缩活动 

分 类 号：R962.2[医药卫生—药理学]